Optimal time window of myelotomy in rats with acute traumatic spinal cord injury: a preliminary study
Optimal time window of myelotomy in rats with acute traumatic spinal cord injury: a preliminary study
复制标题
DOI：
10.1038/sc.2013.56
复制
发表时间：
2013-06
期刊：
影响因子：
2.2
通讯作者：
De-Gang Yang;Li Jj;R. Gu;Ming-liang Yang;Xin Zhang;L. Du;Wei Sun;F. Gao;Hu Am;Wu Yy;He Jg;Feng Yt;Chu Hy
中科院分区：
文献类型：
--
作者：
De-Gang Yang;Li Jj;R. Gu;Ming-liang Yang;Xin Zhang;L. Du;Wei Sun;F. Gao;Hu Am;Wu Yy;He Jg;Feng Yt;Chu Hy
目的：脊髓损伤(SCI)的病理生理机制部分涉及水肿和血肿的形成。脊髓切开术似乎是一种很有前途的干预措施。然而，脊髓损伤患者合适的脊髓切开术时机尚不清楚。在这里，我们的目的是确定脊髓损伤动物模型的显微手术切开时机。方法：用纽约大学冲击器诱导脊髓损伤模型。成年雌性大鼠65只，随机分为单纯椎板切除组(假手术组)、椎板切除加挫伤组(挫伤组)、椎板切除加挫伤后8、24、48h截髓组(8h-MTG组、24h-MTG组、48h-MTG组)。脊髓损伤后每周通过开阔视野试验和斜面试验评价功能恢复情况。结果：与对照组比较，脊髓损伤后8h和24h截髓明显改善Basso-Beattie-Bresna han评分(P<；0.008)，48h截髓效果较差(P=0.023)。在斜面试验中，所有截骨组的平均角度值均高于对照组(P<；0.005)，并且截骨组的SWMA百分比也高于对照组。脊髓损伤后24 h组大鼠的轴突和髓鞘分数明显高于48 h组(P<；0.005)。结论：脊髓损伤后48 h切断髓鞘可促进大鼠脊髓损伤后的恢复。脊髓损伤后8~24小时可能是脊髓切开的时间窗。
Objectives:Pathophysiological mechanisms underlying spinal cord injury (SCI) partially involve edema and formation of a hematoma. Myelotomy seems to be a promising intervention. However, the appropriate timing of myelotomy is still unknown in SCI. Here we aimed to determine the timing of microsurgical myelotomy in an animal model of SCI.Methods:The SCI model was contusion-induced with a new york university impactor. Sixty-five adult female rats were randomly divided into the following groups: laminectomy alone (the ‘sham group’, SG), laminectomy plus contusion (the ‘contusion group’, CG) or laminectomy plus contusion followed by myelotomy at 8, 24 or 48 h (8 h-MTG [myelotomy-treated group], 24 h-MTG or 48 h-MTG). Functional recovery was evaluated via the open field test and the inclined plane test every week after SCI. The percentage of spared white matter area (SWMA) and ultrastructure characteristics of the injured dorsolateral spinal cord were determined on the 42nd day after SCI.Results:Compared with the CG, myelotomy at 8 h-MTG or 24 h-MTG greatly improved the BASSO-BEATTIE-BRESNAHAN scores (P< 0.008), whereas the 48 h-MTG showed less efficacy (P= 0.023). All myelotomy groups showed higher mean angle values in an inclined plane test (P< 0.005) and had greater percentages of SWMA than the CG. Rats in the 24 h-MTG showed a higher intra-axonal fraction and myelin fraction than those in 48 h-MTG (P< 0.005).Conclusion:Myelotomy up to 48 h after SCI improves recovery in rats. The potential time window of myelotomy may be between 8 and 24 h after SCI.