Diffusion Tensor Imaging of Visual Pathway Abnormalities in Five Glaucoma Animal Models.
Diffusion Tensor Imaging of Visual Pathway Abnormalities in Five Glaucoma Animal Models.
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DOI：
10.1167/iovs.62.10.21
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发表时间：
2021-08-02
影响因子：
4.4
通讯作者：
Chan KC
中科院分区：
文献类型：
--
作者：
Colbert MK;Ho LC;van der Merwe Y;Yang X;McLellan GJ;Hurley SA;Field AS;Yun H;Du Y;Conner IP;Parra C;Faiq MA;Fingert JH;Wollstein G;Schuman JS;Chan KC
使用扩散张量成像 (DTI) 表征五种青光眼动物模型的视觉通路完整性。评估了两种实验诱导的青光眼模型和三种基因确定的青光眼模型。对于诱导模型，通过在成年啮齿动物眼睛中房内注射微珠或对小梁网进行激光光凝来实现慢性眼压升高。对于遗传模型，将色素性青光眼的 DBA/2J 小鼠模型、先天性青光眼的 LTBP2 突变猫模型和正常血压青光眼的转基因 TBK1 小鼠模型与各自基因匹配的健康对照进行比较。沿视神经和视束评估 DTI 参数，包括分数各向异性、轴向扩散率和径向扩散率。除转基因 TBK1 小鼠外，在每种动物模型中均观察到相对于对照小鼠的 IOP 显着升高。与各自的健康对照相比，在微珠和激光诱导的啮齿动物模型、DBA/2J 小鼠和 LTBP2 突变猫的视觉通路上观察到显着较低的分数各向异性和较高的径向扩散率。 DBA/2J 小鼠还表现出较低的轴向扩散率，这在其他检查模型中没有观察到。与对照组相比，转基因 TBK1 小鼠没有观察到明显的 DTI 变化。慢性眼压升高伴随着各向异性分数降低和视神经或视束径向扩散率增加，这表明诱导性和遗传性青光眼动物模型的微结构完整性被破坏。不同模型对轴向扩散率的影响不同，表明该 DTI 指标可能代表病理变化随时间和严重程度变化的不同方面。
To characterize the visual pathway integrity of five glaucoma animal models using diffusion tensor imaging (DTI). Two experimentally induced and three genetically determined models of glaucoma were evaluated. For inducible models, chronic IOP elevation was achieved via intracameral injection of microbeads or laser photocoagulation of the trabecular meshwork in adult rodent eyes. For genetic models, the DBA/2J mouse model of pigmentary glaucoma, the LTBP2 mutant feline model of congenital glaucoma, and the transgenic TBK1 mouse model of normotensive glaucoma were compared with their respective genetically matched healthy controls. DTI parameters, including fractional anisotropy, axial diffusivity, and radial diffusivity, were evaluated along the optic nerve and optic tract. Significantly elevated IOP relative to controls was observed in each animal model except for the transgenic TBK1 mice. Significantly lower fractional anisotropy and higher radial diffusivity were observed along the visual pathways of the microbead- and laser-induced rodent models, the DBA/2J mice, and the LTBP2-mutant cats compared with their respective healthy controls. The DBA/2J mice also exhibited lower axial diffusivity, which was not observed in the other models examined. No apparent DTI change was observed in the transgenic TBK1 mice compared with controls. Chronic IOP elevation was accompanied by decreased fractional anisotropy and increased radial diffusivity along the optic nerve or optic tract, suggestive of disrupted microstructural integrity in both inducible and genetic glaucoma animal models. The effects on axial diffusivity differed between models, indicating that this DTI metric may represent different aspects of pathological changes over time and with severity.
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影响因子：
9.3
作者：
Harder JM;Williams PA;Braine CE;Yang HS;Thomas JM;Foxworth NE;John SWM;Howell GR
通讯作者：
Howell GR
成人青光眼患者和对照组中视神经扩散量张量参数及其与视盘形态和疾病严重程度的相关性。
影响因子：
2
作者：
Chang ST;Xu J;Trinkaus K;Pekmezci M;Arthur SN;Song SK;Barnett EM
通讯作者：
Barnett EM
影响因子：
4.4
作者：
Calkins DJ;Horner PJ
通讯作者：
Horner PJ
DOI：
10.1073/pnas.0913141107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Crish, Samuel D.;Sappington, Rebecca M.;Calkins, David J.
通讯作者：
Calkins, David J.
自噬对DBA/2J自发性青光眼小鼠模型中的眼高血压和神经退行性的贡献。
影响因子：
7
作者：
Hirt J;Porter K;Dixon A;McKinnon S;Liton PB
通讯作者：
Liton PB