PERFUSION DEHYDRATION FIXES ELASTIN AND PRESERVES LUNG AIR-SPACE DIMENSIONS
PERFUSION DEHYDRATION FIXES ELASTIN AND PRESERVES LUNG AIR-SPACE DIMENSIONS
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DOI：
10.1152/jappl.1985.58.1.105
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发表时间：
1985-01-01
影响因子：
3.3
通讯作者：
HOPPIN, FG
中科院分区：
文献类型：
--
作者：
OLDMIXON, EH;SUZUKI, S;HOPPIN, FG
提出了一种保留肺组织用于显微摄影的技术，其空气空间尺寸从新鲜状态保持不变。传统技术是有问题的。由于弹性蛋白的机械固定不充分可能导致变形，因此用分级乙醇血管内灌注使仍然膨胀的肺脱水。犬和兔的肺如此制备有更直的肺泡间隔，更大的空域尺寸和改善的相关性与光散射测量。牛颈韧带（主要是弹性蛋白）仅被戊二醛或四氧化二锇部分固定，但通过脱水可以有效地硬化。灌注脱水有助于忠实地保存实质结构，可能是通过弹性蛋白的机械固定。
A technique to preserve lung tissue for micrography with airspace dimensions unchanged from the fresh state is presented. Conventional techniques were problematical. Since distortion might be caused by inadequate mechanical fixation of elastin, the still-inflated lung was dehydrated by intravascular perfusion with graded ethanols. Canine and rabbit lungs so prepared had straighter alveolar septa, greater airspace dimensions and an improved correlation with light-scattering measurements. Bovine ligamentum nuchae (mostly elastin) was only partially fixed by glutaraldehyde or osmium tetroxide but was effectively stiffened by dehydration. Perfusion dehydration aids in the faithful preservation of parenchymal configuration, probably by mechanical fixation of elastin.