DNA-SYNTHESIS DURING DIVISION CYCLE OF 3 SUBSTRAINS OF ESCHERICHIA-COLI B-R
DNA-SYNTHESIS DURING DIVISION CYCLE OF 3 SUBSTRAINS OF ESCHERICHIA-COLI B-R
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DOI：
10.1016/0022-2836(76)90329-6
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
PIERUCCI, O
中科院分区：
文献类型：
--
作者：
HELMSTETTER, CE;PIERUCCI, O
本文研究了大肠杆菌B r三个亚株（B r A、B r F和B r K）染色体复制与细菌分裂周期的关系。当生长速率大于1.0倍/小时（μ> 1.0）时，三个亚株的一轮染色体复制时间（C）均为42分钟，但一轮染色体复制结束与细胞分裂之间的时间（D）在B r A中为22分钟，在B r F中为16分钟，在B r K中为14分钟。在较慢的增长速度C和D增加，但在三个亚株显着不同的程度。当μ= 0.5时，C和D在B r A中约为80和40分钟，在B r F中约为60和20分钟，在B r K中约为70和20分钟。由于E. coli B r的染色体复制模式不同。最明显的区别是E. coli B r F和E. coli B r K在缓慢生长过程中，在分裂周期的开始和结束时都有一段无DNA合成的时期，而E. coli B r A在循环的最后只包含一个无DNA合成的时期。
The relationship between chromosome replication and the bacterial division cycle has been examined in three substrains of Escherichia coli B r obtained from different sources and designated B r A, B r F and B r K. At growth rates greater than 1.0 doubling per hour (μ> 1.0), the time for a round of chromosome replication (C) was 42 minutes in all three substrains, but the time between the end of a round and cell division (D) was 22 minutes in B r A, 16 minutes in B r F and 14 minutes in B r K. At slower growth rates C and D increased, but to significantly different extents in the three substrains. When μ= 0.5, C and D were approximately 80 and 40 minutes in B r A, 60 and 20 minutes in B r F, and 70 and 20 minutes in B r K. As a consequence of the lengths of the C and D periods in the three stocks of E. coli B r, the patterns of chromosome replication during the division cycle differed. The most obvious difference was that E. coli B r F and E. coli B r K possessed periods devoid of DNA synthesis at both the beginning and the end of the division cycle during slow growth, whereas E. coli B r A contained only one period devoid of DNA synthesis at the end of the cycle.