EP2 receptor stimulation promotes calcium responses in astrocytes via activation of the adenylyl cyclase pathway
EP2 receptor stimulation promotes calcium responses in astrocytes via activation of the adenylyl cyclase pathway
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DOI：
10.1007/s00018-006-6262-9
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发表时间：
2006-11-01
影响因子：
8
通讯作者：
Grohovaz, F.
中科院分区：
文献类型：
--
作者：
Di Cesare, A.;Del Piccolo, P.;Grohovaz, F.
星形胶质细胞是一个异质性的细胞群体，具有多种神经递质和神经调质的受体。最近，前列腺素E-2引起了极大的兴趣，因为它不仅由星形胶质细胞释放，而且还激活与磷脂酶C或腺苷酸环化酶偶联的受体。我们报告说，EP 2受体刺激触发cAMP的生产，但也导致释放的Ca 2+从细胞内存储。这种效应也被类似地偶联到腺苷酸环化酶的其他受体所共享，并通过直接刺激酶或应用cAMP类似物引起。然而，cAMP对Ca 2+反应的刺激不是由蛋白激酶A介导的，因为该激酶的特异性拮抗剂没有作用。在所有星形胶质细胞中未观察到cAMP和Ca 2+之间的这种串扰。因此，它可能反映了一个特定的资源，无论是一个亚群或星形胶质细胞在一个特定的功能状态。
Astrocytes are a heterogeneous population of cells that are endowed with a great variety of receptors for neurotransmitters and neuromodulators. Recently prostaglandin E-2 has attracted great interest since it is not only released by astrocytes but also activates receptors coupled to either phospholipase C or adenylyl cyclase. We report that EP2 receptor stimulation triggers cAMP production but also causes release of Ca2+ from intracellular stores. This effect is shared by other receptors similarly coupled to adenylyl cyclase and elicited by direct stimulation of the enzyme or application of cAMP analogues. However, the stimulation of the Ca2+ response by cAMP is not mediated by protein kinase A, since a specific antagonist of this kinase had no effect. Such a cross-talk between cAMP and Ca2+ was not observed in all astrocytes. It might therefore reflect a specific resource of either a subpopulation or astrocytes in a specific functional state.