Inclusion of a beta-aspartyl peptide bond causes the loss of inherent physiological activities.
Inclusion of a beta-aspartyl peptide bond causes the loss of inherent physiological activities.
复制标题
包含β-天冬氨酰肽键会导致固有生理活性的丧失。
DOI：
10.1093/oxfordjournals.jbchem.a124291
复制
发表时间：
1994
影响因子：
2.7
通讯作者：
Y. Hayakawa
中科院分区：
文献类型：
--
作者：
Y. Hayakawa
生长阻滞肽(GBP)是从粘虫幼虫末龄幼虫血浆中分离得到的一种多肽，由寄生在粘虫体内的小蜂Cotesia kariyai寄生。采用固相合成法，分别以7-荧甲氧羰基(Fmoc)和叔丁氧基(Boc)氨基酸为原料，合成了标准的游离羧基末端GBP酸。用Boc氨基酸合成的多肽具有预期的GBP结构，而用Fmoc氨基酸合成的多肽中约90%是16位带有β-天冬氨酸(Asp)键的多肽。含有β-Asp键的多肽对粘虫幼虫没有任何生理作用。
Growth-blocking peptide (GBP) is a peptide which was initially isolated from the plasma of last instar larvae of the insect armyworm, Pseudaletia separata, parasitized by the parasitoid wasp, Cotesia kariyai. A standard GBP acid, a free carboxyl terminal GBP, was synthesized by solid phase methods, using 7-fluorenylmethoxycarbonyl (Fmoc) and tert-butyloxycarbonyl (Boc) amino acids, respectively. The peptide synthesized using Boc amino acids gave the expected structure of GBP, while approximately 90% of the peptide synthesized using Fmoc amino acids was a peptide with a beta-aspartyl (Asp) bond at position 16. The peptide containing the beta-Asp bond does not exhibit any physiological effect on armyworm larvae.