Complete genome sequence of Tunisvirus, a new member of the proposed family Marseilleviridae
Complete genome sequence of Tunisvirus, a new member of the proposed family Marseilleviridae
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DOI：
10.1007/s00705-014-2023-5
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发表时间：
2014-09-01
影响因子：
2.7
通讯作者：
Colson, Philippe
中科院分区：
文献类型：
--
作者：
Aherfi, Sarah;Boughalmi, Mondher;Colson, Philippe
马赛病毒是马赛病毒科的创始成员，马赛病毒科是第二个发现的感染变形虫的巨型病毒家族。这些病毒已经从环境水样中回收，最近从人类中回收。突尼斯病毒是从突尼斯市的喷泉水中分离出来的，通过在阿米巴属上培养。是一种新的马赛病毒。我们在这里描述它的380，011碱基对基因组。共鉴定出484个蛋白，其中320个和358个分别与马赛病毒和洛桑病毒具有直系同源物（e值<1 e-2），259个和299个分别与马赛病毒和洛桑病毒蛋白具有最佳相互命中。此外，在马赛病毒以外的生物体中发现了144种突尼斯病毒蛋白的显著命中，表明广泛的横向基因转移，正如先前在马赛病毒中所证明的那样。最后，总共确定了21个ORFans。系统发育重建和分析的基因库的马赛病毒，包括直系同源物的比例和平均氨基酸之间的同一性对基因，建议家庭Marseillleviridae包括三个谱系。谱系A由马赛病毒、戛纳8病毒和塞内加尔病毒组成;谱系B由单独的洛桑病毒代表;谱系C具有突尼斯病毒作为其第一成员。综上所述，这些发现表明马赛病毒显示出相当程度的多样性。
Marseillevirus is the founding member of the proposed family Marseilleviridae, which is the second discovered family of giant viruses that infect amoebae. These viruses have been recovered from environmental water samples and, more recently, from humans. Tunisvirus was isolated from fountain water in Tunis, Tunisia, by culturing on Acanthamoeba spp. and is a new marseillevirus. We describe here its 380,011 base-pair genome. A total of 484 proteins were identified, among which 320 and 358 have an ortholog in Marseillevirus and Lausannevirus (e-value < 1e-2), respectively, and 259 and 299 have best reciprocal hits with a Marseillevirus and a Lausannevirus protein, respectively. In addition, a significant hit was found in organisms other than marseilleviruses for 144 Tunisvirus proteins, indicating extensive lateral gene transfers, as has been demonstrated previously for Marseillevirus. Finally, a total of 21 ORFans were identified. Phylogeny reconstructions and analysis of the gene repertoires of marseilleviruses, including the proportion of orthologs and the mean amino acid identity between genes in pairs, suggest that the proposed family Marseilleviridae encompasses three lineages. Lineage A is composed of Marseillevirus, Cannes 8 virus and Senegalvirus; lineage B is represented by Lausannevirus alone; and lineage C has Tunisvirus as its first member. Taken together, these findings suggest that the marseilleviruses display a substantial level of diversity.