First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1)
First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1)
复制标题
DOI：
10.1111/j.1574-695x.2006.00184.x
复制
发表时间：
2007-03-01
影响因子：
--
通讯作者：
Wittenbrink, Max M.
中科院分区：
文献类型：
--
作者：
Hoelzle, Ludwig E.;Hoelzle, Katharina;Wittenbrink, Max M.
猪血营养性支原体是一种猪红细胞的细胞外寄生虫，由于其不可培养性，其抗原结构在很大程度上是未知的。在这项研究中，血清蛋白质组和质谱分析可以表征猪抗体反应所针对的猪支原体蛋白质：两种具有热休克蛋白特征的蛋白质，两种具有糖酵解酶特征的蛋白质，一种RNA解旋酶和一种肌动蛋白样蛋白质。对猪分枝杆菌 (HspA1) 的 DnaK 样蛋白 (HspA1) 进行了进一步的遗传和功能分析。其编码基因（M. suis a1 基因）大小为 1.830 bp，对应于 67 kDa 的蛋白质。免疫电子显微镜验证了 HspA1 在猪分枝杆菌中的表面可及性。在大肠杆菌中表达的重组 HspA1 在实验感染的猪中表现出 ATP 酶活性和抗原性。总之，猪分枝杆菌抗原蛋白的首次鉴定和重组表达为疫苗和新的体外诊断测定的开发提供了基础。
The antigenic structures of the haemotrophic Mycoplasma suis, an epicellular parasite of porcine erythrocytes, are largely unknown due to its unculturability. In this study, serological proteome and mass spectrometry analyses allowed the characterization of M. suis proteins targeted by the porcine antibody response: two proteins with characteristics of heat shock proteins, two proteins with characteristics of glycolytic enzymes, a RNA helicase- and an actin-like protein. The DnaK-like protein of M. suis (HspA1) was further analysed genetically and functionally. Its encoding gene (M. suis a1 gene) is 1.830 bp in size and corresponds to a 67 kDa protein. Immunoelectron microscopy verified the surface accessibility of HspA1 in M. suis. Recombinant HspA1 expressed in Escherichia coli demonstrated ATPase activity and antigenicity in experimentally infected pigs. In conclusion, this first identification and recombinant expression of an antigenic protein of M. suis provides the basis for the development of vaccines and new in vitro diagnostic assays.