Claudin18 associated with corticosteroid-induced expression of surfactant proteins in pulmonary epithelial cells
Claudin18 associated with corticosteroid-induced expression of surfactant proteins in pulmonary epithelial cells
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Claudin18 与皮质类固醇诱导的肺上皮细胞表面活性蛋白表达相关
DOI：
10.1080/14767058.2017.1392505
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Huajing Wan
中科院分区：
文献类型：
--
作者：
Fang Shi;Yong Liao;Youzhi Dong;Yuqin Xie;Huajing Wan
摘要目的：本研究旨在验证Cldn18是否与肺成熟有关，并在糖皮质激素诱导的肺表面活性蛋白表达中起关键作用。方法：收集不同发育阶段(E14.5、E16.5和E18.5)的小鼠胚胎肺组织，用免疫印迹和免疫荧光染色检测Cldn18的时空表达。地塞米松 + cAMP + 异丁基甲基黄嘌呤是一种常用的体外诱导肺泡上皮细胞成熟的药物。收集经DCI处理或不经DCI处理的小鼠肺泡上皮细胞系MLE12，用实时荧光定量RT-PCR检测Cldn18和表面活性物质蛋白的表达。对于Si-RNA实验，在DCI处理前将对照siRNA或Cldn18siRNA导入MLE-12细胞24 h。实时荧光定量RT-PCR检测Cldn18和表面活性物质蛋白的表达。结果：Cldn18在外周肺上皮细胞中有表达，在E14.5~E18.5期间表达增强。DCI处理后MLE12细胞Cldn18和表面活性物质(SPA、SPB和SPC)的表达显著增加。沉默Cldn18可有效抑制DCI诱导的SPB和SPC的表达，但不能抑制SPA的表达。结论：Cldn18的表达与外周肺上皮细胞成熟有关，并在糖皮质激素诱导的肺表面活性蛋白表达中起关键作用。
Abstract Objective: The present study was undertaken to test the hypothesis that if Cldn18 is associated with lung maturation and play a key role in the corticosteroid-induced expression of surfactant proteins. Methods: Mouse embryonic lung tissue at different developmental stages (E14.5, E16.5 and E18.5) was collected to detect the temporal-spatial expression of Cldn18 by Western blot and immunofluorescence staining. Dexamethasone + cAMP + isobutylmethylxanthine (DCI), a commonly used agent for inducing maturation of alveolar epithelial cell in vitro, was used to mimic the roles of corticosteroid. Mouse alveolar epithelial cell line MLE12 treated with or without DCI were harvested, expression of Cldn18 and surfactant proteins were assessed by real-time RT-PCR. For Si-RNA experiments, MLE-12 was transfected with control siRNA or Cldn18 siRNA for 24 h before DCI treatment. Expression of Cldn18 and surfactant proteins was assessed by real-time RT-PCR. Results: Expression of Cldn18 was detected in peripheral lung epithelial cells, and increased expression was detected from E14.5 to E18.5 during lung development. Expression of Cldn18 and surfactants (SPA, SPB and SPC) were significantly induced in MLE12 after DCI treatment. Silencing of Cldn18 effectively suppressed the DCI-induced expression of SPB and SPC but not SPA. Conclusion: Our results demonstrated that expression of Cldn18 is associated with peripheral lung epithelial cell maturation and play a key role in the corticosteroid-induced expression of surfactant proteins.
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DOI：
10.1152/ajplung.00127.2002
发表时间：
2002-11-01
影响因子：
4.9
作者：
Gonzales, LW;Guttentag, SH;Ballard, PL
通讯作者：
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影响因子：
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作者：
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影响因子：
3.6
作者：
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通讯作者：
Aagaard, Kjersti
DOI：
10.1016/j.bbalip.2005.09.002
发表时间：
2005-10-15
影响因子：
4.8
作者：
Gunasekara, L;Sch端rch, S;Amrein, M
通讯作者：
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