Nanobiocatalysis for protein digestion in proteomic analysis.
Nanobiocatalysis for protein digestion in proteomic analysis.
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DOI：
10.1002/pmic.200900519
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发表时间：
2010-02
期刊：
影响因子：
3.4
通讯作者：
Smith, Richard D.
中科院分区：
文献类型：
--
作者：
Kim, Jungbae;Kim, Byoung Chan;Lopez-Ferrer, Daniel;Petritis, Konstantinos;Smith, Richard D.
在自下而上的蛋白质组学分析中，蛋白质消化过程是成功鉴定蛋白质的关键步骤。为了取代目前长期、繁琐、难以自动化的溶液中蛋白质消化方法，人们一直致力于开发一种快速、可循环、自动化的消化系统。纳米生物催化方法的最新进展通过使用各种纳米材料，如纳米多孔材料、磁性纳米颗粒和聚合物纳米纤维，提高了蛋白质消化的性能。特别是，胰蛋白酶包被纳米纤维形式的胰蛋白酶稳定取得了前所未有的成功，在重复使用一年的情况下，胰蛋白酶活性没有下降，并且保持了良好的蛋白质水解能力，这表明它在自动化、高通量和在线消化系统的开发中具有巨大的潜力。本文综述了纳米生物催化方法在提高蛋白质消化速度、检测灵敏度、可回收性和胰蛋白酶稳定性方面的最新进展。此外，我们还介绍了非常规能量输入下蛋白质变性的蛋白质消化和微流控酶反应器的发展，这些微流控酶反应器可以受益于这些纳米生物催化方法的最新成功。
The process of protein digestion is a critical step for successful protein identification in the bottom-up proteomic analysis. To substitute the present practice of in-solution protein digestion, which is long, tedious, and difficult to automate, a lot of efforts have been dedicated for the development of a rapid, recyclable and automated digestion system. Recent advances of nanobiocatalytic approaches have improved the performance of protein digestion by using various nanomaterials such as nanoporous materials, magnetic nanoparticles, and polymer nanofibers. Especially, the unprecedented success of trypsin stabilization in the form of trypsin-coated nanofibers, showing no activity decrease under repeated uses for one year and retaining good resistance to proteolysis, has demonstrated its great potential to be employed in the development of automated, high-throughput, and on-line digestion systems. This review discusses recent developments of nanobiocatalytic approaches for the improved performance of protein digestion in speed, detection sensitivity, recyclability, and trypsin stability. In addition, we also introduce the protein digestions under unconventional energy inputs for protein denaturation and the development of microfluidic enzyme reactors that can benefit from recent successes of these nanobiocatalytic approaches.
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影响因子：
3.4
作者：
Kim, Byoung Chan;Lopez-Ferrer, Daniel;Lee, Sang-Mok;Ahn, Hye-Kyung;Nair, Sujith;Kim, Seong H.;Kim, Beom Soo;Petritis, Konstantinos;Camp, David G.;Grate, Jay W.;Smith, Richard D.;Koo, Yoon-Mo;Gu, Man Bock;Kim, Jungbae
通讯作者：
Kim, Jungbae
影响因子：
4.1
作者：
Dormady, SJ;Lei, JM;Regnier, FE
通讯作者：
Regnier, FE
影响因子：
2.9
作者：
Bilkova, Zuzana;Slovakova, Marcela;Viovy, Jean-Louis
通讯作者：
Viovy, Jean-Louis
影响因子：
2
作者：
Jeng, Jingyueh;Lin, Mon-Fang;Shiea, Jentaie
通讯作者：
Shiea, Jentaie
影响因子：
4.4
作者：
Feng, S;Ye, ML;Zou, HF
通讯作者：
Zou, HF