Detection of autoantibodies against the pituitary-specific proteins in patients with lymphocytic hypophysitis.
Detection of autoantibodies against the pituitary-specific proteins in patients with lymphocytic hypophysitis.
复制标题
淋巴细胞性垂体炎患者垂体特异性蛋白自身抗体的检测。
DOI：
10.1530/eje.0.1470767
复制
发表时间：
2002
影响因子：
5.8
通讯作者：
N. Amino
中科院分区：
文献类型：
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作者：
Susumu Tanaka;K. Tatsumi;M. Kimura;T. Takano;Y. Murakami;T. Takao;Kozo Hashimoto;Y. Kato;N. Amino
目的
有几份报告描述了淋巴细胞性垂体炎患者通过免疫荧光或免疫印迹法检测抗垂体抗体。然而，除了脑垂体激素外，尚未研究脑垂体特异性的单个抗原。为了了解淋巴细胞性垂体炎的发病机制，并有效地诊断这种疾病，我们研究了存在的自身抗体对三种垂体特异性蛋白质，生长激素和两种新的垂体特异性蛋白质，即垂体特异性因子1a（PGSF1a）和PGSF2。
设计
淋巴细胞性垂体炎17例，均伴有垂体增大（淋巴细胞性腺垂体炎5例，淋巴细胞性漏斗神经垂体炎12例，其中后一组3例经活检证实），14例垂体功能减退但无垂体增大10例单纯ACTH缺乏症和4例特发性TSH缺乏症，并与11例无功能垂体大腺瘤进行比较，31例其他自身免疫性疾病患者和36例健康对照。
方法
使用重组人（35）S标记蛋白，通过放射性配体测定研究每种抗体的存在。
结果
三名（18%）淋巴细胞性垂体炎患者垂体肿大，五名（36%）垂体功能减退症患者垂体肿大和三名（9.7%）其他自身免疫性疾病的患者是一个或多个抗体阳性的研究。
结论
淋巴细胞性垂体炎和其他垂体功能减退症患者中检测到抗人GH、抗PGSF 1a和抗PGSF 2抗体，但在无功能垂体大腺瘤患者中未检测到。这些抗体的检测可能有助于淋巴细胞性垂体炎的诊断。
OBJECTIVE
Several reports have described antipituitary antibodies by immunofluorescent or immunoblotting methods in patients with lymphocytic hypophysitis. However, with the exception of the pituitary hormones, individual antigens specific for the pituitary gland have not been studied. To understand the pathogenesis of lymphocytic hypophysitis and to diagnose this disease efficiently, we studied the presence of autoantibodies against three pituitary-specific proteins, GH and two novel pituitary-specific proteins, namely, pituitary gland specific factor 1a (PGSF1a) and PGSF2.
DESIGN
Seventeen patients with lymphocytic hypophysitis, all of whom had pituitary enlargement (5 with lymphocytic adenohypophysitis and 12 with lymphocytic infundibuloneurohypophysitis, including 3 of the latter group proven by biopsy), and 14 patients with hypopituitarism without pituitary enlargement (10 with isolated ACTH deficiency and 4 with idiopathic TSH deficiency) were studied, and compared with 11 patients with non-functioning pituitary macroadenoma, 31 patients with other autoimmune diseases, and 36 healthy controls.
METHODS
The presence of each antibody was studied by radioligand assay using recombinant human (35)S-labeled protein.
RESULTS
Three (18%) patients with lymphocytic hypophysitis having pituitary enlargement, five (36%) patients with hypopituitarism without pituitary enlargement and three (9.7%) patients with other autoimmune diseases were positive for one or more of the antibodies studied.
CONCLUSIONS
Anti-human GH, anti-PGSF1a, and anti-PGSF2 antibodies were detected in patients with lymphocytic hypophysitis and other hypopituitarism, but were not detected in patients with non-functioning pituitary macroadenoma. Detection of these antibodies may be useful for the diagnosis of lymphocytic hypophysitis.
影响因子：
--
作者：
Daniel J. Bernard;Teresa K Woodruff
通讯作者：
Daniel J. Bernard;Teresa K Woodruff