Tissue-engineered spiral nerve guidance conduit for peripheral nerve regeneration
Tissue-engineered spiral nerve guidance conduit for peripheral nerve regeneration
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DOI：
10.1016/j.actbio.2018.04.046
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发表时间：
2018-06-01
影响因子：
9.7
通讯作者：
Yu, Xiaojun
中科院分区：
文献类型：
--
作者：
Chang, Wei;Shah, Munish B.;Yu, Xiaojun
最近在周围神经再生方面，临床前研究表明，使用具有多个纵向通道和腔内地形的神经引导导管（NGC）可以增强桥接创伤性损伤引起的神经间隙时的功能结果。这些特征不仅为神经再生提供了指导线索，而且成为开发新型NGC的必要途径。在这项研究中，首先开发并研究了一种新型螺旋NGC，其具有排列的纳米纤维并包裹有外部纳米纤维管。使用常见的大鼠坐骨10毫米神经缺损模型，体内研究表明，新型螺旋NGC（有或没有内部纳米纤维）在恢复6周后提高了神经再生的成功率。根据步行轨迹分析、电生理学、神经组织学评估和腓肠肌测量的结果，将螺旋NGC与内部纳米纤维和外部纳米纤维管相结合，实现了神经再生的实质性改善。这表明，与标准管状 NGC 相比，具有内部对齐纳米纤维并包裹有外部纳米纤维管的新型螺旋 NGC 为周围神经再生提供了更好的环境。这项研究的结果将有利于未来的NGC设计，以优化周围神经再生的组织工程策略。意义声明我们开发了一种新型螺旋神经引导导管（NGC），其带有涂层对齐纳米纤维。相对于管状 NGC，螺旋结构的表面积增加了 4.5 倍。此外，排列好的纳米纤维涂覆在螺旋壁上，为引导神经突延伸提供线索。最后，螺旋NGC的外部随机包裹纳米纤维，以增强机械强度，从而稳定螺旋NGC。我们的神经组织学数据表明，在大鼠坐骨神经 10 毫米间隙中，螺旋 NGC 的有髓轴突比管状结构多 50%，可实现神经再生。这项研究的结果有助于进一步优化周围神经修复的组织工程策略。 (C) 2018 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Recently in peripheral nerve regeneration, preclinical studies have shown that the use of nerve guidance conduits (NGCs) with multiple longitudinally channels and intra-luminal topography enhance the functional outcomes when bridging a nerve gap caused by traumatic injury. These features not only provide guidance cues for regenerating nerve, but also become the essential approaches for developing a novel NGC. In this study, a novel spiral NGC with aligned nanofibers and wrapped with an outer nanofibrous tube was first developed and investigated. Using the common rat sciatic 10-mm nerve defect model, the in vivo study showed that a novel spiral NGC (with and without inner nanofibers) increased the successful rate of nerve regeneration after 6 weeks recovery. Substantial improvements in nerve regeneration were achieved by combining the spiral NGC with inner nanofibers and outer nanofibrous tube, based on the results of walking track analysis, electrophysiology, nerve histological assessment, and gastrocnemius muscle measurement. This demonstrated that the novel spiral NGC with inner aligned nano fibers and wrapped with an outer nanofibrous tube provided a better environment for peripheral nerve regeneration than standard tubular NGCs. Results from this study will benefit for future NGC design to optimize tissue-engineering strategies for peripheral nerve regeneration.Statement of SignificanceWe developed a novel spiral nerve guidance conduit (NGC) with coated aligned nanofibers. The spiral structure increases surface area by 4.5 fold relative to a tubular NGC. Furthermore, the aligned nanofibers was coated on the spiral walls, providing cues for guiding neurite extension. Finally, the outside of spiral NGC was wrapped with randomly nanofibers to enhance mechanical strength that can stabilize the spiral NGC. Our nerve histological data have shown that the spiral NGC had 50% more myelinated axons than a tubular structure for nerve regeneration across a 10 mm gap in a rat sciatic nerve. Results from this study can help further optimize tissue engineering strategies for peripheral nerve repair. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.