An enzymatic‐histochemical study of the corpus uteri of the mouse
An enzymatic‐histochemical study of the corpus uteri of the mouse
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小鼠子宫体的酶组织化学研究
DOI：
10.1002/ar.1091460313
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发表时间：
1963
期刊：
影响因子：
--
通讯作者：
R. Willighagen
中科院分区：
文献类型：
--
作者：
M. Thiery;R. Willighagen
作者研究了C3H/N小鼠子宫体各组成部分中15种酶的分布规律，其中有些酶是首次发现的。在子宫内膜上皮碱性和酸性磷酸酶、5-核苷酸酶、三磷酸腺苷酶、β-葡萄糖醛酸苷酶、NADD、NADPD和所研究的脱氢酶中，发现其强度和定位呈现周期性变化，与雌激素注入动物平行。在子宫内膜间质中发现碱性磷酸酶、三磷酸腺苷和β-葡萄糖苷酸酶活性的周期性变化。在子宫肌层中，碱性磷酸酶和β-葡萄糖苷酸酶也有类似的变化。在上皮下结缔组织中，成纤维细胞的功能活性与碱性磷酸酶的活性强度之间存在一定的关系。巨噬细胞表现出多种酶系统的活性。它们的高酸性磷酸酶活性是由于储存了来自子宫内膜上皮破裂的有机物质。怀疑β-葡萄糖醛酸酶的酶活性和储存功能之间存在类似的关系。动情周期末期子宫内膜上皮和间质中氨基肽酶活性的突然升高可能是这一阶段强烈分解代谢现象的表现。
The authors have investigated in the component parts of the uterine corpus of the C3H/N mouse the distribution pattern of 15 enzymes, some of them for the first time. In the endometrial epithelium alkaline and acid phosphatase, 5‐nucleotidase, adenosinetriphosphatase, β‐glucuronidase, NADD, NADPD, and the dehydrogenases studied, were found to show cyclical variations in intensity and localization which parallel the estrogen impregnation of the animal. Cyclical variations in intensity were found in the endometrial stroma for the activity of alkaline phosphatase, adenosinetriphosphatase, and β‐glucuronidase. Analogous variations in the myometrium are reported for alkaline phosphatase and β‐glucuronidase. A relationship between the functional activity of fibroblasts and the intensity of the activity of alkaline phosphatase is suspected in the subepithelial connective tissue. Macrophages show activity of a variety of enzyme systems. Their high acid phosphatase activity is to be attributed to storage of organic substances deriving from the breakdown of the endometrial epithelium. A similar relationship between enzyme activity and storage function is suspected for β‐glucuronidase. The sudden increase in the aminopeptidase activity in the endometrial epithelium and stroma at the end of the estrous cycle may be the expression of the intense catabolic phenomena which characterize this phase.