Subcellullar localization of tumor-associated antigen 3H11Ag
Subcellullar localization of tumor-associated antigen 3H11Ag
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DOI：
10.1016/j.bbrc.2004.09.133
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发表时间：
2004-11-12
影响因子：
3.1
通讯作者：
Shou, CC
中科院分区：
文献类型：
--
作者：
Guo, JH;Jin, GL;Shou, CC
3H11Ag是一种由单克隆抗体3H11定义的肿瘤相关抗原，可特异性识别各种肿瘤组织中的癌细胞，最近被成功克隆，但其功能尚不清楚。为了探讨它在肿瘤中的潜在作用，我们分析了它的亚细胞定位。通过在COS-7细胞中表达与荧光蛋白融合的3H11Ag，我们发现3H11Ag在细胞质和细胞核中都有定位，亚细胞分离证实了这一点。对转染3H11Ag的COS-7细胞的细胞核进行序列提取，发现它是一种DNA和核基质相关蛋白。此外，通过表达一系列红色荧光蛋白标记的3H11Ag的截短形式，证明其c端的150个氨基酸残基完全负责亚细胞定位。此外，3H11Ag的计算分析结果与实验分析结果一致。这些数据将有助于阐明3H11Ag的功能。(C) 2004爱思唯尔公司版权所有。
3H11Ag, a tumor-associated antigen defined by the monoclonal antibody 3H11 that specifically recognizes cancer cells in various tumor tissues, was successfully cloned recently, but its function is unknown. To explore the potential roles it plays in tumors, we analyzed its subcellular localization in the present study. By expressing 3H11Ag fused with fluorescent protein in COS-7 cells, we found that 3H11Ag localizes to both cytoplasm and nucleus, which was confirmed by subcellular fractionation. And sequentially extracting the nuclei of COS-7 cells transfected with 3H11Ag showed that it is a DNA- and nuclear matrix-associated protein. Moreover, by expressing a series of red fluorescent protein-tagged truncated forms of 3H11Ag, it was demonstrated that the 150 amino acid residues at its C-terminal are fully responsible for the subcellular localization. In addition, the results of the computational analysis of 3H11Ag were in accordance with those of the experimental analysis. All these data would be helpful to elucidate the functions of 3H11Ag. (C) 2004 Elsevier Inc. All rights reserved.