Separability of calcium slow waves and functional connectivity during wake, sleep, and anesthesia.
Separability of calcium slow waves and functional connectivity during wake, sleep, and anesthesia.
复制标题
DOI：
10.1117/1.nph.6.3.035002
复制
发表时间：
2019-07-01
期刊：
影响因子：
5.3
通讯作者：
Culver, Joseph P
中科院分区：
文献类型：
--
作者：
Brier, Lindsey M;Landsness, Eric C;Culver, Joseph P
大脑状态的调节（例如通过麻醉）会改变自发活动的相关结构，尤其是在 Delta 波段。这种效应很大程度上归因于 1 Hz 缓慢振荡，这是麻醉和非快速眼动 (NREM) 睡眠的特征。然而，慢振荡对相关结构和跨大脑状态（包括 NREM）自发活动频谱内容的影响尚未得到全面研究。此外，血红蛋白与钙 (GCaMP6) 成像观察到的活动动态之间的差异尚未得到调和。最后，慢振荡是否取代了警报状态中典型的功能连接（FC）模式，或者叠加在它们之上，仍不清楚。在这里，我们使用广域钙成像来研究清醒、麻醉和自然睡眠小鼠的自发皮质活动。我们发现 infraslow 相关性存在适度的大脑状态依赖性变化，但 GCaMP6 delta 相关性变化较大。对 Delta 波段 GCaMP6 睡眠/麻醉数据的主成分分析表明，慢振荡主要局限于前三个成分。去除这些成分揭示了与尾流期间观察到的惊人相似的相关结构。这些结果表明，在 NREM 睡眠/麻醉期间，缓慢振荡叠加到规范的 FC 架构上。
Modulation of brain state, e.g., by anesthesia, alters the correlation structure of spontaneous activity, especially in the delta band. This effect has largely been attributed to the 1 Hz slow oscillation that is characteristic of anesthesia and nonrapid eye movement (NREM) sleep. However, the effect of the slow oscillation on correlation structures and the spectral content of spontaneous activity across brain states (including NREM) has not been comprehensively examined. Further, discrepancies between activity dynamics observed with hemoglobin versus calcium (GCaMP6) imaging have not been reconciled. Lastly, whether the slow oscillation replaces functional connectivity (FC) patterns typical of the alert state, or superimposes on them, remains unclear. Here, we use wide-field calcium imaging to study spontaneous cortical activity in awake, anesthetized, and naturally sleeping mice. We find modest brain state-dependent changes in infraslow correlations but larger changes in GCaMP6 delta correlations. Principal component analysis of GCaMP6 sleep/anesthesia data in the delta band revealed that the slow oscillation is largely confined to the first three components. Removal of these components revealed a correlation structure strikingly similar to that observed during wake. These results indicate that, during NREM sleep/anesthesia, the slow oscillation superimposes onto a canonical FC architecture.