In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina.
In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina.
复制标题
DOI：
10.2147/ijn.s44885
复制
发表时间：
2013
影响因子：
8
通讯作者：
Morita Y
中科院分区：
文献类型：
--
作者：
Song Y;Ju Y;Song G;Morita Y
细胞的黏附、迁移和增殖受到细胞培养基质表面形貌的显著影响。氧化铝是骨科应用最广泛的植入材料之一，但关于在纳米孔结构上生长的间充质干细胞(MSCs)的细胞反应的数据很少。将MSCs分别培养在光滑氧化铝和纳米多孔氧化铝上，研究纳米多孔氧化铝表面形貌与细胞行为之间的相互作用。以孔径为2 0 nm和10 0 nm的纳米氧化铝为载体，从细胞增殖、细胞形态、整合素β1表达和成骨分化等方面评价孔径对骨髓间充质干细胞的影响。采用四甲基偶氮唑盐比色法测定细胞在不同基质上的存活率，发现细胞存活率随孔径的增大而降低。用扫描电子显微镜研究了孔径对细胞形态的影响。在孔径较大的氧化铝上培养的细胞可见极长的细胞和明显的细胞膜突起。在多孔氧化铝载体上培养的骨髓间充质干细胞中整合素β1的表达增强，表明多孔氧化铝基质比光滑氧化铝基质更有利于细胞生长。成骨细胞分化标志物，如碱性磷酸酶、骨钙素和矿化，在100 nm氧化铝上培养的细胞比在20 nm孔或光滑氧化铝上培养的细胞高。这项工作表明细胞的行为受到孔径大小变化的影响，为这种新型生物相容性材料在组织工程领域的潜在应用提供了新的视角。
Cell adhesion, migration, and proliferation are significantly affected by the surface topography of the substrates on which the cells are cultured. Alumina is one of the most popular implant materials used in orthopedics, but few data are available concerning the cellular responses of mesenchymal stem cells (MSCs) grown on nanoporous structures. MSCs were cultured on smooth alumina substrates and nanoporous alumina substrates to investigate the interaction between surface topographies of nanoporous alumina and cellular behavior. Nanoporous alumina substrates with pore sizes of 20 nm and 100 nm were used to evaluate the effect of pore size on MSCs as measured by proliferation, morphology, expression of integrin β1, and osteogenic differentiation. An MTT assay was used to measure cell viability of MSCs on different substrates, and determined that cell viability decreased with increasing pore size. Scanning electron microscopy was used to investigate the effect of pore size on cell morphology. Extremely elongated cells and prominent cell membrane protrusions were observed in cells cultured on alumina with the larger pore size. The expression of integrin β1 was enhanced in MSCs cultured on porous alumina, revealing that porous alumina substrates were more favorable for cell growth than smooth alumina substrates. Higher levels of osteoblastic differentiation markers such as alkaline phosphatase, osteocalcin, and mineralization were detected in cells cultured on alumina with 100 nm pores compared with cells cultured on alumina with either 20 nm pores or smooth alumina. This work demonstrates that cellular behavior is affected by variation in pore size, providing new insight into the potential application of this novel biocompatible material for the developing field of tissue engineering.