Molecular architecture of the human specialised atrioventricular conduction axis
Molecular architecture of the human specialised atrioventricular conduction axis
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DOI：
10.1016/j.yjmcc.2010.12.017
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发表时间：
2011-04-01
影响因子：
5
通讯作者：
Dobrzynski, H.
中科院分区：
文献类型：
--
作者：
Greener, I. D.;Monfredi, O.;Dobrzynski, H.
房室传导轴位于房室交界处的间隔部分，可以说是心脏中最复杂的结构。它具有多种功能，包括在房室收缩和后备起搏之间引入延迟。像任何其他多功能组织一样，复杂性是心脏这种特殊组织的一个关键特征，这种复杂性既是解剖学的，也是电生理学的，两者密不可分。我们用定量聚合酶链式反应、组织学和免疫组织化学的方法对6名受试者的轴突进行了分析。在心房肌(AM)、移行区(TA)、下结节延伸(INE)、致密结节(CN)、穿透束(PB)和心室肌(Vm)中，检测了与50个离子和缝隙连接通道类似的钙处理蛋白和标志物。与AM相比，CN中Na(V)1.5、K(Ir)12.1、Cx43和ANP mRNAs的表达较低，而HCN1、HCN4、Ca(V)1.3、Ca(V)3.1、K(Ir)3.4、Cx40和TBX3 mRNAs的表达较高。一些相关蛋白的表达与相应的mRNAs的表达一致。在人的房室传导轴上，离子和缝隙连接通道以及钙处理蛋白的表达具有复杂和异质性，这解释了这一重要途径的功能。(C)2011爱思唯尔有限公司。保留所有权利。
The atrioventricular conduction axis, located in the septal component of the atrioventricular junctions, is arguably the most complex structure in the heart. It fulfils a multitude of functions, including the introduction of a delay between atrial and ventricular systole and backup pacemaking. Like any other multifunctional tissue, complexity is a key feature of this specialised tissue in the heart, and this complexity is both anatomical and electrophysiological, with the two being inextricably linked. We used quantitative PCR, histology and immunohistochemistry to analyse the axis from six human subjects. mRNAs for similar to 50 ion and gap junction channels, Ca2+-handling proteins and markers were measured in the atrial muscle (AM), a transitional area (TA), inferior nodal extension (INE), compact node (CN), penetrating bundle (PB) and ventricular muscle (VM). When compared to the AM, we found a lower expression of Na(v)1.5, K(ir)12.1, Cx43 and ANP mRNAs in the CN for example, but a higher expression of HCN1, HCN4, Ca(v)1.3, Ca(v)3.1, K(ir)3.4, Cx40 and Tbx3 mRNAs. Expression of some related proteins was in agreement with the expression of the corresponding mRNAs. There is a complex and heterogeneous pattern of expression of ion and gap junction channels and Ca2+-handling proteins in the human atrioventricular conduction axis that explains the function of this crucial pathway. (C) 2011 Elsevier Ltd. All rights reserved.