A new rapid method for genetic typing of human immunoglobulins.
A new rapid method for genetic typing of human immunoglobulins.
复制标题
人类免疫球蛋白基因分型的一种新的快速方法。
作者：
Girish N. Vyas;H. Fudenberg;H. M. Pretty;E. Gold
本文报道了一种用CrCl_3法将已知基因型的γ-球蛋白和骨髓瘤蛋白包被于人O型细胞上的被动血凝技术。这样包被的细胞已成功地用于人血清的Gm和Inv分型。抑制性和非抑制性血清之间的区别程度与使用不完全抗Rh包被的细胞的常规方法一样大。
A passive hemagglutination technique employing isolated γ-globulins and myeloma proteins of known genetic types coated onto human group O cells by CrCl3 method has been developed. Cells thus coated have been successfully used for Gm and Inv typing of human sera. The degree of discrimination between inhibiting and noninhibiting sera is as great as with the conventional method using cells coated with incomplete anti-Rh.