Cloning and characterization of ribosomal protein S29, a deltamethrin resistance associated gene from Culex pipiens pallens
Cloning and characterization of ribosomal protein S29, a deltamethrin resistance associated gene from Culex pipiens pallens
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淡色库蚊溴氰菊酯抗性相关基因核糖体蛋白 S29 的克隆和表征
DOI：
10.1007/s00436-011-2443-z
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发表时间：
2011-12-01
影响因子：
2
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
--
作者：
Sun, Haibo;Sun, Linchun;Zhu, Changliang
40S核糖体蛋白S29(RPS29)在淡色库蚊对溴氰菊酯(DM)敏感和抗性中有差异表达。在这里，我们克隆了这只蚊子的RPS29。该基因全长171bp，编码56个氨基酸残基，与其他蚊虫RPS29基因有90%以上的同源性。此外，实时定量聚合酶链式反应分析表明，RPS29的转录水平在抗DM的CX中显著上调。Pallens和C6/36细胞与敏感细胞比较。在DM敏感的C6/36细胞中，RPS29的过表达使DM敏感的C6/36细胞的存活率略有下降，而siRNA下调RPS29的表达则相对提高了DM耐药细胞的存活率。我们的研究首次提供了RPS29可能与蚊子抵抗DM的适合度成本相关的证据。
The 40S ribosomal protein S29 (RPS29) was shown differentially expressed in deltamethrin (DM)-susceptible and resistant Culex pipiens pallens previously. Here, we cloned RPS29 from this mosquito. An open reading frame (ORF) of 171 bp was found to encode a putative protein with 56 amino acid residues, which shares more than 90% identities with other mosquito RPS29 genes. Moreover, quantitative real-time PCR analysis demonstrated that the transcriptional level of RPS29 was significantly up-regulated both in DM-resistant Cx. p. pallens and C6/36 cells compared with the susceptible ones. Over-expression of RPS29 showed a slight decrease of cell viability in DM-susceptible C6/36 cells under DM treatment and knockdown of RPS29 by siRNA comparably increased cell viability in DM-resistant cells. Our study provides evidence that RPS29 might be associated with fitness cost of DM resistance in mosquitoes for the first time.