Immune responses during human schistosomiasis mansoni. VI. In vitro nonspecific suppression of phytohemagglutinin responsiveness induced by exposure to certain schistosomal preparations.
Immune responses during human schistosomiasis mansoni. VI. In vitro nonspecific suppression of phytohemagglutinin responsiveness induced by exposure to certain schistosomal preparations.
复制标题
人类曼氏血吸虫病期间的免疫反应。
作者：
D. Colley;C. Todd;F. Lewis;R. Goodgame
人外周血单个核细胞同时暴露于植物血凝素-P(PHA)和曼氏血吸虫可溶性虫卵抗原制剂(SEA)或可溶性尾虫抗原制剂(CAP)时，与单独暴露PHA相比，其反应性降低。加入可溶性成虫抗原制剂(SWAP)并不能预测地改变该体系中的PHA反应。无论淋巴细胞捐献者是曼氏葡萄球菌患者(早期感染、慢性感染或治疗)还是未感染的受试者，体外暴露于SEA或CAP都会表现出抑制作用。抑制的程度与所用SEA的浓度和暴露时间有关。在加入PHA前预先暴露于海洋中3天，可产生更强的抑制作用。然而，在PHA暴露后6小时和24小时加入SEA的时间延迟，其抑制能力分别降低和消除。SEA和CAP对反应细胞没有直接毒性，似乎通过T淋巴细胞相关机制发挥其非特异性抑制作用。我们观察到，虽然这些抑制事件可以在体外诱导和观察，但曼氏血吸虫患者的淋巴细胞对PHA的反应性与未感染的对照组相同。
Simultaneous in vitro exposure of human peripheral blood mononuclear cells to phytohemagglutinin-P (PHA) and either soluble schistosomal egg antigenic preparation (SEA) or soluble cercarial antigenic preparation (CAP) obtained from Schistosoma mansoni resulted in decreased responsiveness as compared to exposure to PHA alone. The addition of a soluble adult worm antigenic preparation (SWAP) did not predictably alter PHA responses in this system. The suppression due to in vitro exposure to either SEA or CAP was expressed whether the lymphocyte donors were S. mansoni patients (early infection, chronic, or treated), or uninfected subjects. The degree of suppression was related to the concentration of SEA used, and the timing of exposure. Preexposure to SEA for 3 days before the addition of PHA resulted in more potent suppression. However, a delay in the time of the addition of SEA of 6 and 24 hr after PHA exposure decreased and eliminated, respectively, its suppressive capacity. SEA and CAP were not directly toxic to responding cells, and appeared to exert their nonspecific suppressive influences through T lymphocyte-related mechanisms. It was observed that although these suppressive events could be induced and observed in vitro, the responsiveness of S. mansoni patient lymphocytes to PHA was equal with that of uninfected controls.