Mechanical properties and collagen content differ between isolated guinea pig duodenum, jejunum, and distal ileum
Mechanical properties and collagen content differ between isolated guinea pig duodenum, jejunum, and distal ileum
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DOI：
10.1023/a:1018855113849
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发表时间：
1998-09-01
影响因子：
3.1
通讯作者：
Gregersen, H
中科院分区：
文献类型：
--
作者：
Storkholm, JH;Villadsen, GE;Gregersen, H
我们比较了豚鼠十二指肠，空肠和回肠远端的分离段的应力-应变分布，以及弹性特性和胶原含量之间的关系。将节段浸入含有10（-2）M MgCl 2的Krebs-Ringer溶液中以消除收缩活性。测量横截面积（CSA）的腔内球囊的逐步膨胀提供了腔内压力加载刺激。通过20 MHz A型超声测量壁厚。应力-应变和增量弹性模量-应变分布来自于壁厚、内半径和施加压力的稳态值。CSA与压力、管壁厚度与压力的关系均为非线性关系，各节段间均存在差异（P < 0.01）。应力-应变分布呈指数分布，符合方程Y = a。实验（B . X）（r（2）= 0.97 +/- 0.01）。与y轴的截距（a）和曲线斜率（B）在节段之间不同（P < 0.01和P < 0.05）。十二指肠、空肠和回肠远端的胶原蛋白含量分别为3.99 +/- 0.18 μ g/mg、2.51 +/- 0.13 μ g/mg和2.10 +/- 0.11 μ g/mg。三个地点之间差异显著（P > 0.05）。在70 kPa应力水平下，胶原含量与弹性模量（刚度）增量之间存在相关性（P < 0.05）。
We compared the stress-strain distributions obtained from isolated segments of the guinea pig duodenum, jejunum, and distal ileum, and the relation between the elastic properties and the collagen content. The segments were immersed in Krebs-Ringer solution containing 10(-2) M MgCl2 to abolish contractile activity. Stepwise inflation of an intraluminal balloon in which the cross-sectional area (CSA) was measured provided the luminal pressure-loading stimulus. The wall thickness was measured by means of 20-MHz A-mode ultrasound. The stress-strain and the incremental elastic modulus-strain distributions were derived from the steady-state values of wall thickness, internal radius, and applied pressure. The CSA-pressure relations and the wall thickness-pressure relations were nonlinear and both differed between the segments (P < 0.01). The stress-strain distributions showed an exponential behavior that fitted well to the equation Y = a . Exp(b . X) (r(2) = 0.97 +/- 0.01). The intercept with the y axis (a) and the slope of the curves (b) differed between the segments (P < 0.01 and P < 0.05). The collagen contents were 3.99 +/- 0.18 mu g/mg, 2.51 +/- 0.13 mu g/mg, and 2.10 +/- 0.11 mu g/mg in the duodenum, jejunum, and distal ileum, respectively. This difference was significant among all three locations (P > 0.05). An association was found between the collagen content and the incremental elastic modulus (stiffness) at a stress level of 70 kPa (P < 0.05).