Novel Degradable Co-polymers of Polypyrrole Support Cell Proliferation and Enhance Neurite Out-Growth with Electrical Stimulation
Novel Degradable Co-polymers of Polypyrrole Support Cell Proliferation and Enhance Neurite Out-Growth with Electrical Stimulation
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DOI：
10.1163/092050609x12481751806330
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发表时间：
2010-01-01
影响因子：
3.6
通讯作者：
Schmidt, Christine E.
中科院分区：
文献类型：
--
作者：
Durgam, Hymavathi;Sapp, Shawn;Schmidt, Christine E.
合成聚合物，如聚吡咯（PPy），由于其导电性能，在神经研究中越来越重要。我们评估了两种新型的聚吡咯与聚（ε-己内酯）（PCL）和聚（氰基丙烯酸乙酯）（PECA）的生物可降解嵌段共聚物用于神经再生的应用。PPy-PCL和PPy-PECA共聚物可以由溶剂基胶体分散体加工而成，并且与PPy均聚物（22 S/cm）相比具有基本上相同或更大的电导率（PPy-PCL为32 S/cm，PPy-PECA为19 S/cm）。共聚物的PPy部分允许电刺激，而PCL或PECA嵌段能够通过水解降解。对于体外测试，通过空气刷涂分散体层并压制膜在聚碳酸酯片材上制备膜。我们的特点是水解降解，导电性，细胞增殖和神经突延伸的电影。共聚物足以进行细胞的电刺激，而不需要在共聚物膜下面的金属导体。在体外电刺激的PPy-PCL显着增加了PC 12细胞轴承神经突起的数量相比，未受刺激的PPy-PCL。对于体内实验，将PPy共聚物涂覆到由市售的非导电生物可降解聚合物聚（3-羟基丁酸酯-共-3-羟基戊酸酯）（PHB-HV）制成的神经引导通道（NGC）的内壁上。将NGCs植入大鼠坐骨神经中的10 mm缺损中，并在8周后收获。组织学染色显示轴突生长。研究表明，这些新型导电可降解生物材料具有良好的生物相容性，并支持体外PC 12细胞（有和无电刺激）和体内神经元（无电刺激）的增殖和生长。(C)Koninklijke Brill NV，莱顿，2010年
Synthetic polymers such as polypyrrole (PPy) are gaining significance in neural studies because of their conductive properties. We evaluated two novel biodegradable block co-polymers of PPy with poly(epsilon-caprolactone) (PCL) and poly(ethyl cyanoacrylate) (PECA) for nerve regeneration applications. PPy-PCL and PPy-PECA co-polymers can be processed from solvent-based colloidal dispersions and have essentially the same or greater conductivity (32 S/cm for PPy-PCL, 19 S/cm for PPy-PECA) compared to the PPy homo-polymer (22 S/cm). The PPy portions of the co-polymers permit electrical stimulation whereas the PCL or PECA blocks enable degradation by hydrolysis. For in vitro tests, films were prepared on polycarbonate sheets by air brushing layers of dispersions and pressing the films. We characterized the films for hydrolytic degradation, electrical conductivity, cell proliferation and neurite extension. The co-polymers were sufficient to carry out electrical stimulation of cells without the requirement of a metallic conductor underneath the co-polymer film. In vitro electrical stimulation of PPy-PCL significantly increased the number of PC12 cells bearing neurites compared to unstimulated PPy-PCL. For in vivo experiments, the PPy co-polymers were coated onto the inner walls of nerve guidance channels (NGCs) made of the commercially available non-conducting biodegradable polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV). The NGCs were implanted in a 10 mm defect made in the sciatic nerve of rats, and harvested after 8 weeks. Histological staining showed axonal growth. The studies indicated that these new conducting degradable biomaterials have good biocompatibility and support proliferation and growth of PC12 cells in vitro (with and without electrical stimulation) and neurons in vivo (without electrical stimulation). (C) Koninklijke Brill NV, Leiden, 2010