Activation-dependent redistribution of the adhesion plaque protein, talin, in intact human platelets.
Activation-dependent redistribution of the adhesion plaque protein, talin, in intact human platelets.
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DOI：
10.1083/jcb.109.6.3333
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发表时间：
1989-12
影响因子：
7.8
通讯作者：
Locke, S J
中科院分区：
文献类型：
--
作者：
Beckerle, M C;Miller, D E;Bertagnolli, M E;Locke, S J
Talin是一种高相对分子质量的蛋白质，定位于成纤维细胞的黏附斑块。它结合纽蛋白和整合素，似乎参与了细胞外基质和细胞骨架之间的跨膜连接。我们最近发现，Talin是一种在血小板中含量丰富的蛋白质，血小板是高度专用于调节黏附的细胞。尽管Talin在完整的人血小板中占总蛋白的3%以上，但它在细胞内的位置尚未确定。在本文报道的工作中，我们用免疫荧光和免疫电子显微镜研究了塔林在静息和激活的人血小板中的分布。我们发现Talin在其亚细胞位置经历了依赖于激活的改变。静息状态下的血小板是非粘附性的，塔林均匀地分布在细胞质中。相反，在凝血酶和玻璃激活的基质黏附的血小板中，Talin集中在质膜的细胞质表面。这种戏剧性的、受调控的Talin重新分布增加了Talin在受控的血小板黏附发展中发挥作用的可能性。
Talin is a high molecular weight protein localized at adhesion plaques in fibroblasts. It binds vinculin and integrin and appears to participate in generating a transmembrane connection between the extracellular matrix and the cytoskeleton. We have recently shown that talin is an abundant protein in platelets, cells highly specialized for regulated adhesion. Although talin constitutes greater than 3% of the total protein in intact human platelets, its location within the cells had not been defined. In the work reported here, we have investigated the distribution of talin in resting and activated human platelets by immunofluorescence and immunoelectron microscopy. We have found that talin undergoes an activation-dependent change in its subcellular location. In resting platelets, which are nonadhesive, talin is uniformly distributed throughout the cytoplasm. In contrast, in thrombin- and glass-activated, substratum-adherent platelets, talin is concentrated at the cytoplasmic face of the plasma membrane. This dramatic, regulated redistribution of talin raises the possibility that talin plays a role in the controlled development of platelet adhesion.