One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples

One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples
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DOI:
10.1128/cdli.10.3.476-478.2003
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发表时间:
2003-05-01
期刊:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子:
--
通讯作者:
Chanteau, S
Chanteau, S
中科院分区:
其他
文献类型:
--
作者:
Nato, F;Boutonnier, A;Chanteau, S

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我们描述了一种快速诊断试验的发展和评价霍乱弧菌01和0139的基础上脂多糖检测金颗粒。特异性范围为84 - 100%。当用在马达加斯加和孟加拉国获得的粪便样本进行评价时,试纸的灵敏度范围为94.2%至100%。试纸可以为流行病学调查提供一个简单的工具。
We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae 01 and 0139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.