Culturing of glial and neuronal cells on polysialic acid
Culturing of glial and neuronal cells on polysialic acid
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DOI：
10.1016/j.biomaterials.2006.10.030
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发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Grothe, C.
中科院分区：
文献类型：
--
作者：
Haile, Y.;Haastert, K.;Grothe, C.
虽然周围神经在横断损伤后表现出再生能力，但神经修复的成功关键取决于差距的长度。除了自体神经移植作为传统的神经外科治疗，以克服长的差距，替代策略是必要的。已经进行了许多实验研究，以找到用于生产人工假体的最佳材料，这些假体可以作为神经残端之间的管道引入。目前的研究遵循的目标是建立聚唾液酸（polySia），α 2，8-连接的唾液酸残基的均聚物，作为一种新型的，生物相容性和生物可吸收的神经组织工程材料。作为实现这一目标的第一步，建立了用可溶性polySia有效包被细胞培养皿的方案。此外，在polySia基质上培养作为重建治疗候选物的原代神经细胞，包括新生儿和成人许旺细胞、神经祖细胞、脊神经节神经元和运动神经元。评价培养物的细胞存活和细胞增殖能力。polySia在细胞培养条件下是稳定的，诱导的可降解产物和降解产物对细胞培养物没有负面影响。此外，polySia揭示了它的兼容性，从大鼠胚胎，出生后和成年神经组织来源的几种细胞类型时，用作基板。(c)2006爱思唯尔有限公司保留所有权利。
Although peripheral nerves exhibit regeneration capacities after transection injuries, the success of nerve repair depends crucially on the length of the gap. In addition to autologous nerve grafting as the conventional neurosurgical treatment to overcome long gaps, alternative strategies are needed. Numerous experimental studies have been undertaken to find the optimal material for production of artificial prostheses, which can be introduced as conduits between the nerve stumps. The current study follows the aim to establish polysialic acid (polySia), a homopolymer of alpha 2,8-linked sialic acid residues, as a novel, biocompatible, and bioresorbable material for nerve tissue engineering. As a first step towards this goal, protocols for efficient coating of cell culture dishes with soluble polySia were established. In addition, primary nerve cells which are candidates for reconstructive therapies, including neonatal and adult Schwann cells, neural progenitor cells, spinal ganglionic neurons and motoneurons were cultured on polySia substrates. Cultures were evaluated with regard to cell survival and cell proliferation capacities. polySia turned out to be stable under cell culture conditions, and induced degradable and degradation products had no negative effects on cell cultures. Furthermore, polySia revealed its compatibility for several cell types derived from rat embryonic, postnatal and adult nervous tissue when used as a substrate. (c) 2006 Elsevier Ltd. All rights reserved.