In vivo stability and biocompatibility of implanted calcium alginate disks
In vivo stability and biocompatibility of implanted calcium alginate disks
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DOI：
10.1002/jbm.a.31275
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发表时间：
2007-12-15
影响因子：
4.9
通讯作者：
Kipke, Daryl R.
中科院分区：
文献类型：
--
作者：
Nunamaker, Elizabeth A.;Purcell, Erin K.;Kipke, Daryl R.
藻酸盐是一种常用的生物医学水凝胶，其在体内的降解行为才刚刚开始被了解。藻酸盐在中枢神经系统中作为电极涂层、细胞包封基质以及用于硬脑膜修补术的应用越来越广泛。然而，有必要了解水凝胶在体内的行为，以帮助开发藻酸盐作为神经接口材料。本研究的目的是比较植入后取出的藻酸盐圆盘的流变行为以及在3个月期间皮下植入藻酸盐水凝胶所引起的炎症反应。具体而言，研究了（1）原位胶凝、（2）扩散胶凝以及（3）使用多聚-L -赖氨酸（PLL）涂层所产生的影响。虽然所有样品的复数模量在第一天都下降了80%，但原位胶凝的藻酸盐在植入的第一周更为稳定。PLL涂层使扩散胶凝的藻酸盐稳定性有所提高，但两种条件下的稳定性仍显著低于原位胶凝的藻酸盐。在生物相容性方面没有差异能明确表明一种胶凝方法优于另一种。这些结果表明，在以稳定性为首要考虑因素的神经接口应用中，原位胶凝是首选方法。（c）2007威利期刊公司
Alginate is a commonly used biomedical hydrogel whose in vivo degradation behavior is only beginning to be understood. The use of alginate in the central nervous system is gaining popularity as an electrode coating, cell encapsulation matrix, and for duraplasty. However, it is necessary to understand how the hydrogel will behave in vivo to aid in the development of alginate for use as a neural interface material. The goal of the current study was to compare the rheological behavior of explanted alginate disks and the inflammatory response to subcutaneously implanted alginate hydrogels over a 3-month period. Specifically, the effects due to (1) in situ gelling, (2) diffusion gelling, and (3) use of a poly-L-lysine (PLL) coating were investigated. While all samples' complex moduli decreased 80% in the first day, in situ gelled alginate was more stable for the first week of implantation. The PLL coating offered some stability increases for diffusion gelled alginate, but the stability in both conditions remained significantly lower than that in in situ gelled alginate. There were no differences in biocompatibility that clearly suggested one gelation method over another. These results indicate that in situ gelation is the preferred method in neural interface applications where stability is the primary concern. (c) 2007 Wiley Periodicals, Inc.