The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut
The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut
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DOI：
10.1038/ng.3517
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发表时间：
2016-04-01
期刊：
影响因子：
30.8
通讯作者：
Ozias-Akins, Peggy
中科院分区：
文献类型：
--
作者：
Bertioli, David John;Cannon, Steven B.;Ozias-Akins, Peggy
栽培花生（Arachis hypogaea）是一种异源四倍体，其近缘亚基因组的总大小约为2.7 Gb。这使得染色体假分子的组装非常具有挑战性。作为了解栽培花生基因组的基础，我们报道了其二倍体祖先（Arachis duranensis和Arachis ipaensis）的基因组序列。我们发现这些基因组与栽培花生的A和B亚基因组相似，并利用它们来鉴定候选抗病基因，指导四倍体转录本组装和检测栽培花生亚基因组之间的遗传交换。根据A. ipaensis基因组和栽培花生的B亚基因组的序列比较，并结合植物地理学证据，我们认为A. ipaensis可能是将B亚基因组贡献给栽培花生的同一种群的直系后代。
Cultivated peanut (Arachis hypogaea) is an allotetraploid with closely related subgenomes of a total size of similar to 2.7 Gb. This makes the assembly of chromosomal pseudomolecules very challenging. As a foundation to understanding the genome of cultivated peanut, we report the genome sequences of its diploid ancestors (Arachis duranensis and Arachis ipaensis). We show that these genomes are similar to cultivated peanut's A and B subgenomes and use them to identify candidate disease resistance genes, to guide tetraploid transcript assemblies and to detect genetic exchange between cultivated peanut's subgenomes. On the basis of remarkably high DNA identity of the A. ipaensis genome and the B subgenome of cultivated peanut and biogeographic evidence, we conclude that A. ipaensis may be a direct descendant of the same population that contributed the B subgenome to cultivated peanut.