Cutting edge:: Dendritic cells are essential for in vivo IL-12 production and development of resistance against Toxoplasma gondii infection in mice
Cutting edge:: Dendritic cells are essential for in vivo IL-12 production and development of resistance against Toxoplasma gondii infection in mice
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DOI：
10.4049/jimmunol.177.1.31
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发表时间：
2006-07-01
影响因子：
4.4
通讯作者：
Aliberti, Julio
中科院分区：
文献类型：
--
作者：
Liu, Cheng-Hu;Fan, Yu-ting;Aliberti, Julio
一个强大的IFN-γ反应被触发后感染的原生动物寄生虫，弓形虫。包括树突细胞（DO）、巨噬细胞和嗜中性粒细胞在内的几种细胞群产生IL-12，IL-12是IFN-γ诱导的关键细胞因子。然而，目前还不清楚上述细胞群中的哪一个是其主要来源。白喉毒素（DT）注射导致瞬时DC消耗在转基因小鼠表达猿猴DT受体的CD 11 c启动子的控制下，使我们能够调查的作用，DC在IL-12的生产。T.监测刚地氏菌接种的DT处理组和对照组的IL-12水平和存活率。我们在该研究中显示DC消耗消除了IL-12的产生并导致死亡。此外，补充野生型，但不是MyD 88-或IL-12 p35-缺陷，DC拯救IL-12的生产，IFN-γ诱导，并抵抗DC-耗尽小鼠的感染。综上所述，本研究中呈现的结果表明，在T.弓形虫感染
A powerful IFN-gamma response is triggered upon infection with the protozoan parasite, Toxoplasma gondii. Several cell populations, including dendritic cells (DO), macrophages, and neutrophils, produce IL-12, a key cytokine for IFN-gamma induction. However, it is still unclear which of the above cell populations is its main source. Diphtheria toxin (D T) injection causes transient DC depletion in a transgenic mouse expressing Simian DT receptors under the control of the CD11c promoter, allowing us to investigate the role of DCs in IL-12 production. T. gondii-inoculated DT-treated and control groups were monitored for IL-12 levels and survival We show in this study that DC depletion abolished IL-12 production and led to mortality. Furthermore, replenishment with wild-type, hut not MyD88- or IL-12p35-deficient, DCs rescued IL-12 production, IFN-gamma-induction, and resistance to infection in DC-depleted mice. Taken together, the results presented in this study indicate that DCs constitute the major IL12-producing cell population in vivo during T. gondii infection.