Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α Functionally Improves the Uterus Repair
Local Delivery of Silk-Cellulose Incorporated with Stromal Cell-Derived Factor-1α Functionally Improves the Uterus Repair
复制标题
局部输送与基质细胞衍生因子 1 α 结合的丝纤维素可在功能上改善子宫修复
DOI：
10.1089/ten.tea.2018.0283
复制
发表时间：
2019-09-03
影响因子：
4.1
通讯作者：
Zou, Xiaohui
中科院分区：
文献类型：
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作者：
Cai, Hongxia;Wu, Bingbing;Zou, Xiaohui
子宫的严重感染和机械性损伤可能导致不孕和流产。目前，子宫损伤的功能修复缺乏有效的治疗方式。为了应对这一临床挑战，本研究旨在通过将重组人基质细胞衍生因子-1α（rhSDF-1α）整合到丝素蛋白-细菌纤维素（SF-BC）膜载体中来开发趋化复合支架。本研究采用大鼠子宫损伤模型，分为三组：空白对照组、仅植入SF-BC、或加载rhSDF-1α的SF-BC。分析比较三组的组织再生效果。结果表明，装载rhSDF-1α的SF-BC显着增强受损大鼠子宫的子宫内膜再生和动脉生成，从而改善妊娠结局，从而表明功能性子宫修复和再生的前景广阔。影响陈述在这项研究中，我们证明丝素蛋白-细菌纤维素（SF-BC）膜在体外具有良好的物理、化学和生物相容性。体内研究表明，SF-BC膜中掺入重组人基质细胞衍生因子1α（rhSDF-1α）可促进全层子宫损伤的再生，并改善受损子宫的妊娠结局。因此，结果表明负载rhSDF-1α的SF-BC在未来子宫损伤修复的临床应用中具有良好的潜力。
Severe infection and mechanical injury of the uterus may lead to infertility and miscarriage. Currently, there is a lack of effective treatment modality for functional repair of uterine injury. To address this clinical challenge, this study aimed to develop a chemotactic composite scaffold by incorporating recombinant human stromal cell-derived factor-1 alpha (rhSDF-1 alpha) into a silk fibroin-bacterial cellulose (SF-BC) membrane carrier. A rat model of uterine injury was utilized for this study, which was composed of three groups as follows: blank control, implantation with SF-BC only, or SF-BC loaded with rhSDF-1 alpha. The tissue regeneration efficacy of the three groups was analyzed and compared. The results showed that SF-BC loaded with rhSDF-1 alpha significantly enhanced endometrial regeneration and arteriogenesis of the injured rat uterus, which led to improved pregnancy outcomes, thus indicating much promise for functional uterine repair and regeneration. Impact Statement In this study, we demonstrated that the silk fibroin-bacterial cellulose (SF-BC) membrane possessed good physical, chemical, and biocompatibility properties in vitro. The in vivo study showed that the incorporation of recombinant human stromal cell-derived factor-1 alpha (rhSDF-1 alpha) within the SF-BC membrane promoted regeneration of full-thickness uterine injury and also improved the pregnancy outcome of the damaged uterus. The results thus suggest that SF-BC loaded with rhSDF-1 alpha has good potential in future clinical applications for the repair of uterine injury.