Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients.
Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients.
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DOI：
10.1186/s13287-017-0572-8
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发表时间：
2017-05-16
影响因子：
7.5
通讯作者：
Sekiya I
中科院分区：
文献类型：
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作者：
Kohno Y;Mizuno M;Ozeki N;Katano H;Komori K;Fujii S;Otabe K;Horie M;Koga H;Tsuji K;Matsumoto M;Kaneko H;Takazawa Y;Muneta T;Sekiya I
来源于滑膜的间充质干细胞（滑膜间充质干细胞）由于其高的软骨形成能力而成为软骨和滑膜再生医学的候选细胞来源。再生医学有望用于炎症控制良好的RA患者以及OA患者，滑膜间充质干细胞移植也将是一种可能的治疗方法。滑膜间充质干细胞的某些特性因患者的疾病而异，RA的病理状态是否影响滑膜间充质干细胞的软骨形成仍存在争议。本研究的目的是比较RA和OA患者的原代滑膜间充质干细胞的特性。在全膝关节置换术中，分别从8例RA和OA患者的膝关节中采集人类滑膜组织。滑膜有核细胞培养14天。分析原代滑膜间充质干细胞的总细胞产量、表面标志物和分化潜能。RA和OA中每1 mg滑膜的有核细胞数分别为8.4 ± 3.9千和8.0 ± 0.9千。14天培养后的总细胞数/1 mg滑膜在RA中为0.7 ± 0.4百万，在OA中为0.5 ± 0.3百万，显示RA和OA之间无显著差异。培养14 d后RA和OA细胞大多数为CD 44、CD 73、CD 90、CD 105阳性，CD 45阴性。RA和OA的软骨颗粒重量和每个颗粒的sGAG含量无显著差异。RA和OA的油红O阳性菌落率和茜素红阳性菌落率相似。RA中原代滑膜间充质干细胞的产量、表面标志物和软骨形成潜力与OA中的相当。类风湿关节炎患者的滑膜组织可以作为骨髓间充质干细胞的细胞来源，用于软骨和半月板的再生。本文的在线版本（doi：10.1186/s13287-017-0572-8）包含补充材料，可供授权用户使用。
Mesenchymal stem cells derived from the synovial membrane (synovial MSCs) are a candidate cell source for regenerative medicine of cartilage and menisci due to their high chondrogenic ability. Regenerative medicine can be expected for RA patients with the inflammation well-controlled as well as OA patients and transplantation of synovial MSCs would also be a possible therapeutic treatment. Some properties of synovial MSCs vary dependent on the diseases patients have, and whether or not the pathological condition of RA affects the chondrogenesis of synovial MSCs remains controversial. The purpose of this study was to compare the properties of primary synovial MSCs between RA and OA patients. Human synovial tissue was harvested during total knee arthroplasty from the knee joints of eight patients with RA and OA respectively. Synovial nucleated cells were cultured for 14 days. Total cell yields, surface markers, and differentiation potentials were analyzed for primary synovial MSCs. Nucleated cell number per 1 mg synovium was 8.4 ± 3.9 thousand in RA and 8.0 ± 0.9 thousand in OA. Total cell number after 14-day culture/1 mg synovium was 0.7 ± 0.4 million in RA and 0.5 ± 0.3 million in OA, showing no significant difference between in RA and OA. Cells after 14-day culture were mostly positive for CD44, CD73, CD90, CD105, negative for CD45 both in RA and OA. There was no significant difference for the cartilage pellet weight and sGAG content per pellet between in RA and OA. Both oil red O-positive colony rate and alizarin red-positive colony rate were similar in RA and OA. Yields, surface markers and chondrogenic potential of primary synovial MSCs in RA were comparable to those in OA. Synovium derived from RA patients can be the cell source of MSCs for cartilage and meniscus regeneration. The online version of this article (doi:10.1186/s13287-017-0572-8) contains supplementary material, which is available to authorized users.