Highlighting chromosome loops in DNA-picked chromatin (DPC)
Highlighting chromosome loops in DNA-picked chromatin (DPC)
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DOI：
10.4161/epi.6.8.16060
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发表时间：
2011-08-01
期刊：
影响因子：
3.7
通讯作者：
Perillo, Bruno
中科院分区：
文献类型：
--
作者：
Abbondanza, Ciro;De Rosa, Caterina;Perillo, Bruno
越来越多的证据支持这样的概念，即特定基因座之间的动态染色体内和染色体间联系有助于创建细胞类型特异性基因表达谱。因此，分析控制转录过程的位点之间的特殊功能相关性的建立似乎具有根本的相关性，这些位点在线性DNA上也是遥远的。我们在这里提出了一个实验方法，表明17 β-雌二醇诱导的转录协会之间的启动子和终止区的环的形成的死亡反应基因。该策略揭示了作为一种工具，也适合使用，与自动化技术相结合，用于广泛分析多个基因共享的诱导表达位点。
Growing evidence supports the concept that dynamic intra-and inter-chromosomal links between specific loci contribute to the creation of cell type-specific gene expression profiles. Therefore, analysis of the establishment of peculiar functional correlations between sites, also distant on linear DNA, that govern the transcriptional process appears to be of fundamental relevance. We propose here an experimental approach showing that 17 beta-estradiol-induced transcription associates to formation of loops between the promoter and termination regions of hormone-responsive genes. This strategy reveals as a tool to be also suitably used, in conjunction with automated techniques, for an extensive analysis of sites shared by multiple genes for induced expression.