An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery
An effective and biocompatible polyethylenimine based vaginal suppository for gene delivery
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一种有效且生物相容的聚乙烯亚胺阴道栓剂，用于基因传递
DOI：
10.1016/j.nano.2019.03.016
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Wang Hui
中科院分区：
文献类型：
--
作者：
Ren Ci;Li Xiaomin;Mao Lu;Xiong Jinfeng;Gao Chun;Shen Hui;Wang Liming;Zhu Da;Ding Wencheng;Wang Hui
以人乳头瘤病毒（HPV）为靶点的基因治疗是治疗宫颈癌的一种有效方法，而有效的基因导入方法是其临床应用的关键。我们的目的是制定一个新的药物配方，适当的基因传递阴道内。首次研制了一种新型的聚乙烯亚胺阴道栓剂。切片免疫荧光结果证实了体内和体外的递送功效。绿色荧光蛋白（GFP）转基因小鼠局部上皮的荧光淬灭和基因表达降低证明了栓剂的有效靶向潜力。本研究的另一个目的是评价基于PEI的转移的生物相容性。据我们所知，这也是第一个系统、全面地探讨体内毒性的研究。本研究为PEI栓剂在宫颈癌防治中的转化应用提供了新的思路。
Gene therapy targeted human papillomavirus (HPV) is a promising treatment for cervical cancer, and the key for clinical application depends on an effective gene delivery method. Our aim was to formulate a new pharmaceutical formula for appropriate gene delivery intravaginally. For the first time, we here developed a new polyethylenimine (PEI) based vaginal suppository. The sectional immunofluorescence results confirmed the delivery efficacy both in vivo and in vitro. The quenching fluorescence and decreased gene expression in topical epithelium of green fluorescence protein (GFP) transgenic mice demonstrated the efficient targeting potential of the suppository. The other aim of this study was to evaluate the biocompatibility of the PEI based transfer. To our knowledge, this was also the first study to explore the toxicity in vivo systematically and comprehensively. Our study provided novel ideas for the translational application of PEI based suppository to the prevention and treatment of cervical cancer.