Associative DNA methylation changes in children with prenatal alcohol exposure
Associative DNA methylation changes in children with prenatal alcohol exposure
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DOI：
10.2217/epi.15.60
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发表时间：
2015-01-01
期刊：
影响因子：
3.8
通讯作者：
Singh, Shiva M.
中科院分区：
文献类型：
--
作者：
Laufer, Benjamin I.;Kapalanga, Joachim;Singh, Shiva M.
目的：产前酒精暴露（PAE）可导致胎儿酒精谱系障碍（FASD）。先前，我们评估了小鼠模型脑组织中的PAE，但是这些变化是否存在于人类中仍然未知。材料与方法：在本报告中，我们使用450K阵列在6名FASD儿童的口腔拭子中发现了一些相同的DNA甲基化变化。结果：与原钙粘蛋白、谷氨酸突触和河马信号相关的基因发生了变化。在另一个由6名FASD儿童组成的异质复制组中发现了类似的结果。结论：重复的结果表明，出生时患有FASD的儿童具有独特的DNA甲基化缺陷，这可能受到性别和药物暴露的影响。最终，随着未来的临床发展，评估口腔拭子DNA甲基化可以为FASD的诊断提供一种新的策略。
Aim: Prenatal alcohol exposure (PAE) can cause fetal alcohol spectrum disorders (FASD). Previously, we assessed PAE in brain tissue from mouse models, however whether these changes are present in humans remains unknown. Materials & methods: In this report, we show some identical changes in DNA methylation in the buccal swabs of six children with FASD using the 450K array. Results: The changes occur in genes related to protocadherins, glutamatergic synapses, and hippo signaling. The results were found to be similar in another heterogeneous replication group of six FASD children. Conclusion: The replicated results suggest that children born with FASD have unique DNA methylation defects that can be influenced by sex and medication exposure. Ultimately, with future clinical development, assessment of DNA methylation from buccal swabs can provide a novel strategy for the diagnosis of FASD.