Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair
Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair
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DOI：
10.1101/gad.1273105
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发表时间：
2005-07-15
影响因子：
10.5
通讯作者：
Laurent, BC
中科院分区：
文献类型：
--
作者：
Chai, B;Huang, J;Laurent, BC
细胞修复受损DNA的失败可能导致基因组不稳定和癌症。为了有效地修复染色体DNA损伤，修复机制必须在染色质的背景下接近受损的DNA。在这里，我们报告的RSC和Swi/Snf ATP依赖的染色质重塑复合物在双链断裂（DSB）修复中发挥关键作用，特别是通过同源重组（HR）。RSC和Swi/ Snf各自被募集到体内DSB位点，但具有不同的动力学。我们表明，Swi/Snf需要更早，在或之前的链入侵步骤的HR，而RSC需要突触后完成的重组修复事件。
The failure of cells to repair damaged DNA can result in genomic instability and cancer. To efficiently repair chromosomal DNA lesions, the repair machinery must gain access to the damaged DNA in the context of chromatin. Here we report that both the RSC and Swi/Snf ATP-dependent chromatin-remodeling complexes play key roles in double-strand break (DSB) repair, specifically by homologous recombination (HR). RSC and Swi/ Snf are each recruited to an in vivo DSB site but with distinct kinetics. We show that Swi/Snf is required earlier, at or preceding the strand invasion step of HR, while RSC is required following synapsis for completion of the recombinational repair event.