An assessment of covalent grafting of RGD peptides to the surface of a compliant poly(carbonate-urea)urethane vascular conduit versus conventional biological coatings: Its role in enhancing cellular retention
An assessment of covalent grafting of RGD peptides to the surface of a compliant poly(carbonate-urea)urethane vascular conduit versus conventional biological coatings: Its role in enhancing cellular retention
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DOI：
10.1089/107632702760240580
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发表时间：
2002-08-01
影响因子：
--
通讯作者：
Hamilton, G
中科院分区：
文献类型：
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作者：
Krijgsman, B;Seifalian, AM;Hamilton, G
内皮细胞（EC）假体移植的目的是建立一个功能良好的抗血栓形成的EC单层。基底膜蛋白的应用提高了EC在ePTFE移植物上的粘附性。他们添加到一个生物耐用的柔性聚（碳酸脲）聚氨酯接枝（CPU）研究了关于EC粘附。预凝块、纤维连接蛋白、明胶和胶原蛋白被涂在CPU上。RGD肽、肝素、RGD和肝素均与CPU化学键合。用111-铟氧标记的人脐静脉EC (HUVEC) (1.8 x 10（6) EC/cm(2)）涂于原生和改良的CPU上。90分钟后冲洗移植物，测定EC保留量。实验重复了六次。本机CPU上的EC保留率为1.0 +/- 0.2 x 10(5) EC/cm(2)。预凝块、纤维连接蛋白、明胶和胶原蛋白的应用并没有改善EC潴留，分别为0.8 +/- 0.1、0.4 +/- 0.1、0.3 +/- 0.08和0.5 +/- 0.2 × 10(5) EC/cm(2)。结合RGD、肝素以及RGD和肝素均可显著提高EC保留率，分别达到1.9 +/- 0.6、1.7 +/- 0.5和2.6 +/- 0.6 × 10(5) EC/cm(2) （p < 0.01）。RGD和肝素的结合，以及RGD和肝素的结合加速并增强了EC在CPU上的滞留。基底膜蛋白的简单涂层与天然CPU相比没有优势。
The aim of sodding prosthetic grafts with endothelial cells (EC) is to establish a functioning antithrombogenic monolayer of EC. Application of basement membrane proteins improves EC adherence on ePTFE grafts. Their addition to a biodurable compliant poly(carbonateurea)urethane graft (CPU) was studied with respect to EC adherence. Preclot, fibronectin, gelatin, and collagen were coated onto CPU. RGD peptide, heparin, and both RGD and heparin were chemically bonded to CPU. Human umbilical vein EC (HUVEC) labeled with 111-Indium oxine were sodded (1.8 x 10(6) EC/cm(2)) onto native and the modified CPU. The grafts were washed after 90 min and EC retention determined. The experiments were repeated six times. EC retention on native CPU was 1.0 +/- 0.2 x 10(5) EC/cm(2). The application of preclot, fibronectin, gelatin, and collagen did not improve EC retention, which was 0.8 +/- 0.1, 0.4 +/- 0.1, 0.3 +/- 0.08, and 0.5 +/- 0.2 x 10(5) EC/cm(2), respectively. Bonding RGD, heparin, and both RGD and heparin significantly improved EC retention to 1.9 +/- 0.6, 1.7 +/- 0.5, and 2.6 +/- 0.6 x 10(5) EC/cm(2), respectively (p < 0.01). Bonding of RGD, heparin, and both RGD and heparin accelerates and enhances EC retention onto CPU. Simple coating of basement membrane proteins confers no advantage over native CPU.