Reactive oxygen species mediated ginsenoside Rg3- and Rh2-induced apoptosis in hepatoma cells through mitochondrial signaling pathways
Reactive oxygen species mediated ginsenoside Rg3- and Rh2-induced apoptosis in hepatoma cells through mitochondrial signaling pathways
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DOI：
10.1016/j.fct.2012.05.027
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发表时间：
2012-08-01
影响因子：
4.3
通讯作者：
Kang, Hyung-Sub
中科院分区：
文献类型：
--
作者：
Park, Hye-Min;Kim, Shang-Jin;Kang, Hyung-Sub
人参（P. ginseng）在几种癌症模型中具有抗癌作用。人参皂苷是人参中的主要生物活性成分。韩国红参（Korean Red Ginseng，KRG）提取物能有效地杀死多种癌细胞，而人参皂苷Rg 3（GRg 3）和Rh 2（GRh 2）是KRG中主要的人参皂苷。本研究旨在探讨抗肝癌颗粒及其主要皂苷（GRg 3和GRh 2）对人肝癌细胞（Hep 3B）凋亡的影响。KRG、GRg 3和GRh 2对Hep 3B细胞有明显的细胞毒和凋亡作用，表现为细胞活力和线粒体膜电位降低，但LDH释放增加。在Hep 3B细胞凋亡途径中，KRG、GRg 3和GRh 2具有刺激Hep 3B细胞线粒体细胞色素c释放、激活caspase-3和Bax蛋白、抑制Bcl-2蛋白和产生细胞内活性氧的能力。这些结果表明，KRG，GRg 3和GRh 2可能通过直接激活线粒体途径诱导细胞凋亡。(C)2012爱思唯尔有限公司保留所有权利。
Panax ginseng (P. ginseng) has anti-cancer effects in several cancer models. Ginsenosides are the main bioactive components in P. ginseng. Korean red ginseng (KRG) extract can potently kill various cancer cells and ginsenosides Rg3 (GRg3) and Rh2 (GRh2) are the primary ginsenosides in KRG. This study was carried out to examine whether KRG and its primary ginsenosides (GRg3 and GRh2) affect apoptosis of human hepatocellular carcinoma cells (Hep3B). KRG, GRg3 and GRh2 have obvious cytotoxic and apoptotic effects in Hep3B cells as evidenced by a decrease in cell viability and mitochondria membrane potential, but an increase in LDH release. In the mitochondria-mediated apoptosis pathway, KRG, GRg3 and GRh2 have the ability to stimulate the release of mitochondrial cytochrome c, activation of caspase-3 and Bax protein, inhibition of Bcl-2 protein and production of intracellular reactive oxygen species in Hep3B cells. These results suggest that KRG, GRg3 and GRh2 may induce apoptosis by direct activation of the mitochondrial pathway. (C) 2012 Elsevier Ltd. All rights reserved.