Electroosmotic Flow-Based Pump for Liquid Chromatography on a Planar Microchip
Electroosmotic Flow-Based Pump for Liquid Chromatography on a Planar Microchip
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DOI：
10.1021/ac801497r
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发表时间：
2008-11-01
影响因子：
7.4
通讯作者：
Collins, Greg E.
中科院分区：
文献类型：
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作者：
Borowsky, Joseph F.;Giordano, Braden C.;Collins, Greg E.
电渗流泵与位于微芯片无电场区的溶胶-凝胶固定相结合，实现了6种硝基芳烃和硝胺类炸药及其降解产物的液相色谱分离。集成的泵和LC系统在单个石英衬底内制造。泵区由填充有5 μ m多孔二氧化硅珠的直通道（3.0 cm × 230 μ m × 100 μ m）组成。的溶胶-凝胶固定相是来自于前体混合物的甲基三甲氧基和苯乙基tzimethoxysilane和合成的下游，无场区域的微芯片，导致在固定相单块的尺寸为2.6厘米× 230亩× 100亩米。流体动力学的设计考虑进行了讨论，特别是当它们涉及到集成的LC系统的泵。泵和分离性能，其特征在于流速测量，注射，洗脱，分离和检测，指向一个可行的分析化学平台，包括便携式芯片实验室系统的所有预期好处，包括减少样品要求和小包装。
An electroosmotic flow (EOF)-based pump, integrated with a sol-gel stationary phase located in the electric field-free region of a microchip, enabled the separation of six nitroaromatic and nitramine explosives and their degradation products via liquid chromatography (LC). The integrated pump and LC system were fabricated within a single quartz substrate. The pump region consisted of a straight channel (3.0 cm x 230 pm x 100 pm) packed lwith 5-mu m porous silica beads. The sol-gel stationary phase was derived from a precursor mixture of methyltrimethoxy- and phenethyltzimethoxysilanes and was synthesized in the downstream, field-free region of the microchip, resulting in a stationary-phase monolith with dimensions of 2.6 cm x 230 mu m x 100 mu m. Fluid dynamic design considerations are discussed, especially as they relate to integrating the EOF pump with the LC system. Pump and separation performance, as characterized by flow rate measurements, injection, elution, separation, and detection, point to a viable analytical chemistry platform that encompasses all of the benefits expected of portable, laboratory-on-chip systems, including reduced sample requirements and small packaging.