Genomic structure of the human NDUFS8 gene coding for the iron-sulfur TYKY subunit of the mitochondrial NADH:ubiquinone oxidoreductase
Genomic structure of the human NDUFS8 gene coding for the iron-sulfur TYKY subunit of the mitochondrial NADH:ubiquinone oxidoreductase
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DOI：
10.1016/s0378-1119(98)00275-3
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发表时间：
1998-07-17
期刊：
影响因子：
3.5
通讯作者：
Issartel, JP
中科院分区：
文献类型：
--
作者：
de Sury, R;Martinez, P;Issartel, JP
编码人线粒体NADH：泛醌氧化还原酶（复合物I）的TYKY亚基的NDUFS 8基因的结构组织已通过对从粘粒文库克隆的基因组片段进行测序来确定。NDUFS 8基因位于染色体11 q13上，紧邻ALDH 7同种型基因的下游。它的长度约为6 kb，包含7个外显子，大小从51到186 bp不等。三个CCAAT盒序列基序存在于转录起始的上游。Spl和NRFl结合位点基序存在于第一内含子中。该基因的表达是普遍存在的，但主要在心脏和骨骼肌中。二维凝胶电泳后胎盘线粒体中TYKY亚基的免疫检测显示，成熟蛋白的分子量为22 kDa，pi为4.9-5.0。(C)1998 Elsevier Science B. V.保留所有权利。
The structural organization of the NDUFS8 gene coding for the TYKY subunit of the human mitochondrial NADH:ubiquinone oxidoreductase (Complex I) has been determined by sequencing of a genomic fragment cloned from a cosmid library. The NDUFS8 gene is located on chromosome 11q13 immediately downstream of the ALDH7 isoform gene. It spans about 6 kb and contains seven exons ranging in size from 51 to 186 bp. Three CCAAT box sequence motifs are present upstream of the transcription start. Spl and NRF1 binding site motifs are present in the first intron. Expression of the gene is ubiquitous but predominant in heart and skeletal muscle. Immunodetection of the TYKY subunit in placental mitochondria after two-dimensional gel electrophoresis revealed that the mature protein has a molecular mass of 22 kDa and a pi in the range of 4.9-5.0. (C) 1998 Elsevier Science B.V. All rights reserved.