Proteomic analysis of HIV-infected macrophages.
Proteomic analysis of HIV-infected macrophages.
复制标题
DOI：
10.1007/s11481-010-9253-4
复制
发表时间：
2011-03
影响因子：
6.2
通讯作者：
Toro-Nieves, Dianedis
中科院分区：
文献类型：
--
作者：
Melendez, Loyda M.;Colon, Krystal;Rivera, Linda;Rodriguez-Franco, Eillen;Toro-Nieves, Dianedis
单核吞噬细胞（单核细胞、巨噬细胞和小胶质细胞）在对抗包括HIV在内的病原体的先天免疫中发挥重要作用。这些细胞也是中枢神经系统中重要的病毒储存库，并分泌炎症介质和毒素，影响组织环境和周围细胞的功能。在抗逆转录病毒治疗的时代，这些炎症介质的数量减少了。蛋白质组学方法包括表面增强激光解吸电离，凝胶电泳的一维和二维差异，以及液相色谱串联质谱已被用于揭示体外hiv感染的单核细胞，巨噬细胞和小胶质细胞产生的蛋白质。这些方法促进了对HIV复制和神经元损伤新机制的理解。它们也被用于限制HIV复制的组织巨噬细胞，以了解未来治疗的限制机制。本文综述了hiv感染单核吞噬细胞的蛋白质组学研究，并讨论了最近开始应用于该领域的其他蛋白质组学方法。随着蛋白质组学仪器和方法的发展变得更加敏感和定量，未来的研究可能会发现更多的蛋白质，这些蛋白质可以作为诊断或治疗的目标，并揭示新的疾病机制。
Mononuclear phagocytes (monocytes, macrophages, and microglia) play an important role in innate immunity against pathogens including HIV. These cells are also important viral reservoirs in the central nervous system and secrete inflammatory mediators and toxins that affect the tissue environment and function of surrounding cells. In the era of antiretroviral therapy, there are fewer of these inflammatory mediators. Proteomic approaches including surface enhancement laser desorption ionization, one- and two-dimensional difference in gel electrophoresis, and liquid chromatography tandem mass spectrometry have been used to uncover the proteins produced by in vitro HIV-infected monocytes, macrophages, and microglia. These approaches have advanced the understanding of novel mechanisms for HIV replication and neuronal damage. They have also been used in tissue macrophages that restrict HIV replication to understand the mechanisms of restriction for future therapies. In this review, we summarize the proteomic studies on HIV-infected mononuclear phagocytes and discuss other recent proteomic approaches that are starting to be applied to this field. As proteomic instruments and methods evolve to become more sensitive and quantitative, future studies are likely to identify more proteins that can be targeted for diagnosis or therapy and to uncover novel disease mechanisms.
登录
查看更多内容
DOI：
10.4049/jimmunol.0903563
发表时间：
2010-08-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Akhtar LN;Qin H;Muldowney MT;Yanagisawa LL;Kutsch O;Clements JE;Benveniste EN
通讯作者：
Benveniste EN
影响因子：
4.4
作者：
Ancuta P;Liu KY;Misra V;Wacleche VS;Gosselin A;Zhou X;Gabuzda D
通讯作者：
Gabuzda D
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1111/j.1582-4934.2006.tb00407.x
发表时间：
2006-04
影响因子：
5.3
作者：
Dalle-Donne I;Aldini G;Carini M;Colombo R;Rossi R;Milzani A
通讯作者：
Milzani A
影响因子：
3.7
作者：
Brown JN;Kohler JJ;Coberley CR;Sleasman JW;Goodenow MM
通讯作者：
Goodenow MM