59Co solid-state NMR as a new probe for elucidating metal binding in polynucleotides.
59Co solid-state NMR as a new probe for elucidating metal binding in polynucleotides.
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59Co 固态 NMR 作为阐明多核苷酸中金属结合的新探针。
DOI：
10.1021/ja012353j
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发表时间：
2002
影响因子：
15
通讯作者：
Frydman,Lucio
中科院分区：
文献类型：
--
作者：
Grant,ChristopherV;Frydman,Veronica;Harwood,JohnS;Frydman,Lucio
虽然镁具有几种重要的生物化学作用，但由于其不利的光谱特性，直接研究这种离子是很复杂的。这一贡献探索了通过组合[Co(NH_3)_6]_(3+)作为[Mg(H_2O)_6]_(2+)的替代物和高分辨率的固态~(59)Co−作为光谱探针来监测镁核磁共振核酸结合的可能性。这种策略消除了束缚态和自由态之间的快速阳离子交换，同时利用了59Co自旋的优越的核磁共振特性。对相对少量的tRNA进行的实验可以识别对应于不同金属结合环境的共振。这些表征得到了对模型化合物的研究和多核31P−59Co再偶联实验的辅助。
Although magnesium fulfills several essential biochemical roles, direct studies on this ion are complicated by its unfavorable spectroscopic characteristics. This contribution explores the possibility of monitoring magnesium−nucleic acid binding via a combination of [Co(NH3)6]3+as surrogate for [Mg(H2O)6]2+, and of high-resolution solid-state59Co NMR as a spectroscopic probe. Such strategy quenches fast cationic exchanges between bound and free states, while exploiting the superior NMR properties of the59Co spin. Experiments on relatively small amounts of tRNA can then discern resonances corresponding to different metal binding environments. These characterizations were assisted by studies on model compounds and by multinuclear31P−59Co recoupling experiments.