Transcriptome analysis of the irregular shape of shoot apical meristem in dt (dou tou) mutant of Brassica napus L.
Transcriptome analysis of the irregular shape of shoot apical meristem in dt (dou tou) mutant of Brassica napus L.
复制标题
甘蓝型油菜dt（豆头）突变体茎尖分生组织不规则形状的转录组分析。
DOI：
10.1007/s11032-019-0943-1
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发表时间：
2019-02
影响因子：
3.1
通讯作者：
Xiao-Li Tan
中科院分区：
文献类型：
--
作者：
Ke-Ming Zhu;Shuo Xu;Kai-Xia Li;Sheng Chen;Sundus Zafar;Wei Cao;Zheng Wang;Li-Na Ding;Yan-Hua Yang;Yao-Ming Li;Xiao-Li Tan
油菜（Brassica napus L.）是世界上重要的油料作物。为了满足机械化收获和提高产量的需要，非常需要更好地了解控制该作物主要农艺性状的调控网络。本研究获得了一个油菜多主茎自然突变体，命名为dt。dt突变体表现出茎的异常分化，叶片增多，株高降低。表型和组织切片分析表明，dt表型是由茎尖分生组织（SAM）发育异常引起的。参与SAM活性维持、细胞分裂素合成和信号转导的基因在转录水平上表现出很大的变异，这与dt突变体中细胞分裂素的高水平表达有关。这些结果为甘蓝型油菜的育种和生产提供了理想的材料。
Rapeseed (Brassica napus L.) is an important oil crop in the world. In order to fulfill the requirement of mechanized harvesting and raised production, it is highly desirable to gain a better understanding of the regulatory networks controlling the main agronomic traits of this crop. In this study, we obtained a natural mutant of rapeseed with more main stems named as dt (duo tou, meaning more main stems in Chinese). The dt mutant exhibits abnormal differentiation of stems, increased leaves, and decreased plant height. Phenotype and tissue section analysis showed that abnormal development of the shoot apical meristem (SAM) led to the dt phenotype. Genes that participated in SAM activity maintenance, cytokinin biosynthesis, and signal transduction displayed greatly variation at transcriptional level, which was associated with the high level of cytokinin in the dt mutant. These results provide desirable material for improving the breeding and production of Brassica napus.