Effects of K+ channel agonists cromakalim and pinacidil on rat basilar artery smooth muscle cells are mediated by Ca(++)-activated K+ channels.
Effects of K+ channel agonists cromakalim and pinacidil on rat basilar artery smooth muscle cells are mediated by Ca(++)-activated K+ channels.
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K 通道激动剂克罗卡林和吡那地尔对大鼠基底动脉平滑肌细胞的作用是由 Ca(+) 激活的 K 通道介导的。
DOI：
10.1016/s0006-291x(05)81397-x
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发表时间：
1991
影响因子：
3.1
通讯作者：
B. Weir
中科院分区：
文献类型：
--
作者：
N. Stockbridge;H. Zhang;B. Weir
采用全细胞和无细胞的内面向外膜片钳技术，观察了钾通道开放剂吡那地尔和色满卡林对酶法分离的大鼠基底动脉平滑肌细胞的作用。延迟整流和钙依赖性钾电流被确定从全细胞记录。色满卡林和吡那地尔仅增加钙依赖性钾电流。由内而外的膜斑片的记录揭示了一个大电导的电压和钙依赖性钾通道，该通道被Charybdotoxin阻断，但不受ATP < 10 mM的影响。Cromakalim和吡那地尔增加了该通道的开放概率。根据这些结果，我们认为，这些药物，作用于脑动脉平滑肌细胞钾通道，可能是一些有益的治疗脑血管痉挛后蛛网膜下腔出血。
Whole-cell and cell-free inside-out patch-clamp recording techniques were used to examine the actions of potassium channel openers pinacidil and cromakalim in enzymatically isolated smooth muscle cells of rat basilar artery. Delayed rectifier and calcium-dependent potassium currents were identified from the whole-cell recordings. Only the calcium-dependent potassium current was increased by cromakalim and pinacidil. Recordings from inside-out membrane patches revealed a large conductance voltage- and calcium-dependent potassium channel, which was blocked by charybdotoxin but unaffected by ATP < 10 mM. Cromakalim and pinacidil increased the open probability of this channel. On the basis of these results, we suggest that such drugs, acting on cerebral arterial smooth muscle cell potassium channels, may be of some benefit in the treatment of cerebral vasospasm following subarachnoid hemorrhage.
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