Effects of Wolbachia/Cardinium Infection on the Mitochondrial Phylogeny of Oligonychus castaneae (Acari: Tetranychidae)
Effects of Wolbachia/Cardinium Infection on the Mitochondrial Phylogeny of Oligonychus castaneae (Acari: Tetranychidae)
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DOI：
10.1093/jee/toy354
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发表时间：
2018-11
影响因子：
2.2
通讯作者：
H. Sakamoto;R. Suzuki;N. Nishizawa;T. Matsuda;T. Gotoh
中科院分区：
文献类型：
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作者：
H. Sakamoto;R. Suzuki;N. Nishizawa;T. Matsuda;T. Gotoh
摘要许多无脊椎动物体内都有内共生菌。在这些内共生体中，Wolbachia和Cardinium引起了特别的关注，因为这些细菌经常影响宿主的遗传结构和遗传多样性。它们引起各种生殖改变，如细胞质不相容性、孤雌生殖诱导、雄性杀死和雌性化。通过这些改变，它们也会影响宿主的母系遗传细胞器。线粒体DNA可用于无脊椎动物的分子系统发育分析。然而，在沃尔巴克氏体或Cardinium感染的无脊椎动物，基于线粒体DNA的系统发育树往往是不一致的核DNA的基础上。在本研究中，我们确定了沃尔巴克氏体/Cardinium感染状况的45个人口的螨，栗小爪螨Ehara和Gotoh（阿卡里：叶螨科），收集在日本各地。在此基础上，对O.基于线粒体DNA的细胞色素c氧化酶亚基I（COI）基因和核DNA的28 S rRNA基因，阐明Wolbachia和/或Cardinium感染的影响。在450例受检者中，发现106例Wolbachia感染者和250例Cardinium感染者，感染率为79%。未观察到双重感染个体。在28 S树中，几乎所有的种群形成一个单一的群体。在COI树中，O.栗形成四个独立的组，更密切地遵循沃尔巴克氏体/Cardinium感染比地理分布。这些结果表明，内共生菌影响了O.栗树。
Abstract A wide range of invertebrates harbor intracellular endosymbiotic bacteria. Within these endosymbionts, Wolbachia and Cardinium, have been attracting particular attention because these bacteria frequently affect the genetic structure and genetic diversity of their hosts. They cause various reproductive alterations such as cytoplasmic incompatibility, parthenogenesis induction, male-killing, and feminization. Through these alterations, they also affect the maternally inherited organelles of their hosts. Mitochondrial DNA (mtDNA) can be used for molecular phylogenetic analysis of invertebrates. However, in Wolbachia- or Cardinium-infected invertebrates, phylogenetic trees based on mtDNA are often inconsistent with those based on nuclear DNA. In the present study, we determined the Wolbachia/Cardinium infection status of 45 populations of the mite, Oligonychus castaneae Ehara & Gotoh (Acari: Tetranychidae), collected throughout Japan. Then, we compared phylogenetic trees of O. castaneae based on both the cytochrome c oxidase subunit I (COI) gene of mtDNA and the 28S rRNA gene of nuclear DNA to clarify the effects of Wolbachia and/or Cardinium infection. We found 106 Wolbachia-infected individuals and 250 Cardiniuminfected individuals in a total of 450 individuals, indicating an infection rate of 79%. No double-infected individuals were observed. In the 28S tree, almost all populations formed a single group. In the COI tree, O. castaneae formed four separate groups that more closely followed Wolbachia/Cardinium infection than geographic distribution. These results strongly suggest that the endosymbionts affected mitochondrial variation of O. castaneae.