Automatic Generation of Validated Specific Epitope Sets
Automatic Generation of Validated Specific Epitope Sets
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DOI：
10.1155/2015/763461
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发表时间：
2015-01-01
影响因子：
4.1
通讯作者：
Sette, Alessandro
中科院分区：
文献类型：
--
作者：
Carrasco Pro, Sebastian;Sidney, John;Sette, Alessandro
B和T细胞应答的准确测量是研究自身免疫、过敏、对病原体的免疫和宿主-病原体相互作用的有价值的工具，并有助于T细胞疫苗和免疫疗法的设计和评估。在这种情况下，希望阐明一种选择经验证的参考表位组以允许检测T和B细胞的方法。然而，免疫表位数据库（IEDB）中包含的不断增长的信息以及表位测定之间研究的质量和主题的差异使这项任务变得复杂。在这项研究中，我们开发了一种新的方法来自动选择参考表位集根据IEDB采用的分类系统。从所产生的集合中，通过检测来自人供体的离体T细胞反应性来实验验证三个表位集合（EBV、分枝杆菌和登革热）。此外，创建了一个可能在IEDB中实现的网络应用程序，以允许用户生成定制表位集。
Accurate measurement of B and T cell responses is a valuable tool to study autoimmunity, allergies, immunity to pathogens, and host-pathogen interactions and assist in the design and evaluation of T cell vaccines and immunotherapies. In this context, it is desirable to elucidate a method to select validated reference sets of epitopes to allow detection of T and B cells. However, the ever-growing information contained in the Immune EpitopeDatabase (IEDB) and the differences in quality and subjects studied between epitope assays make this task complicated. In this study, we develop a novel method to automatically select reference epitope sets according to a categorization system employed by the IEDB. From the sets generated, three epitope sets (EBV, mycobacteria and dengue) were experimentally validated by detection of T cell reactivity ex vivo from human donors. Furthermore, a web application that will potentially be implemented in the IEDB was created to allow users the capacity to generate customized epitope sets.