CNS region-specific oxytocin receptor expression: Importance in regulation of anxiety and sex behavior
CNS region-specific oxytocin receptor expression: Importance in regulation of anxiety and sex behavior
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DOI：
10.1523/jneurosci.21-07-02546.2001
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发表时间：
2001-04-01
影响因子：
5.3
通讯作者：
McCarthy, MM
中科院分区：
文献类型：
--
作者：
Bale, TL;Davis, AM;McCarthy, MM
催产素受体（OTR）在CNS中差异表达。由于有多种机制，OTR可以转录诱导，我们假设OTR表达的差异可能是由不同的信号转导通路的激活，并可能是控制焦虑和性行为的关键。为了确定这种表达的调节和功能意义，我们在测定行为和催产素受体结合之前，给雌性大鼠输注了蛋白激酶修饰剂。在下丘脑腹内侧核（VMH），催产素受体的雌激素依赖性诱导需要蛋白激酶C激活，催产素注入这里促进女性性行为，但对焦虑没有影响。与此相反，多巴胺控制紧张性催产素受体的表达在中央杏仁核（cAmyg）通过激活蛋白激酶A，催产素注入这里是抗焦虑，但对女性的性行为没有影响。因此，我们已经确定了VMH和cAmyg中OTR的脑区域特异性调节。不同的信号转导途径调节受体的表达和结合在每个大脑区域可能介导催产素发挥这些差异的行为效应的能力。
The oxytocin receptor (OTR) is differentially expressed in the CNS. Because there are multiple mechanisms by which the OTR can be transcriptionally induced, we hypothesized that differences in OTR expression may be explained by activation of distinct signal transduction pathways and may be critical for the control of anxiety and sex behaviors. To determine the regulation and functional significance of this expression, we infused female rats with modifiers of protein kinases before assaying for behavior and oxytocin receptor binding. In the ventromedial nucleus of the hypothalamus (VMH), estrogen-dependent induction of oxytocin receptors required protein kinase C activation, and oxytocin infused here promoted female sex behavior but had no effect on anxiety. In contrast, dopamine controlled tonic oxytocin receptor expression in the central nucleus of the amygdala (cAmyg) through activation of protein kinase A, and oxytocin infused here was anxiolytic but had no effect on female sex behavior. Therefore, we have identified brain region-specific regulation of the OTR in the VMH and cAmyg. Distinct signal transduction pathways regulating receptor expression and binding in each brain region may mediate in part the ability of oxytocin to exert these differential behavioral effects.