Acetylcholine: its significance in controlling ciliary activity of human respiratory epithelium in vitro.
Acetylcholine: its significance in controlling ciliary activity of human respiratory epithelium in vitro.
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乙酰胆碱：其在体外控制人呼吸道上皮纤毛活动的意义。
DOI：
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发表时间：
1959
影响因子：
3.3
通讯作者：
C. R. Allen
中科院分区：
文献类型：
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作者：
G. Corssen;C. R. Allen
在血浆凝块中培养的人呼吸道纤毛上皮的外植体倾向于聚集并形成旋转的球形，可用于灌注室中，以研究各种化学物质对纤毛活动的影响。灌流0.1-1.0%浓度的乙酰胆碱溶液总是增加细胞团的旋转运动。在用氯化乙酰胆碱灌流前，用0.05-0.1%水杨酸乙酯溶液浸泡上皮，可显著增强乙酰胆碱对纤毛活动的影响。0.1%及更强浓度的硫酸阿托品溶液灌流产生短暂的刺激效应，随后逐渐减少旋转运动。0.5%浓度的乙酰胆碱可拮抗硫酸阿托品的抑制作用。被调查的各种物质的行为似乎与乙酰胆碱在启动和维持药物浓度中起关键作用的假设一致。
Explants of human respiratory ciliated epithelium cultured in a plasma clot tend to round up and to form rotating globes which can be employed in perfusion chambers for the study of the effect of various chemicals on ciliary activity. Perfusion of acetylcholine chloride solutions at 0.1–1.0% concentration invariably increased rotatory movement of the cell cluster. Bathing the epithelial explant in 0.05–0.1% eserine salicylate solutions, prior to the perfusion with acetylcholine chloride, markedly enhanced the acetylcholine effect on ciliary activity. Perfusion of atropine sulfate solutions at concentrations of 0.1% and stronger resulted in a short-lasting stimulatory effect, followed by progressive reduction of rotatory motion. The depressant action of atropine sulfate was counteracted by acetylcholine chloride at 0.5% concentration. The behavior of the various substances investigated appears to be consistent with the assumption that acetylcholine plays the key role in initiating and maintaining the contr...