Perinatal cocaine exposure decreases the number of spontaneously active midbrain dopamine neurons in neonatal rats.
Perinatal cocaine exposure decreases the number of spontaneously active midbrain dopamine neurons in neonatal rats.
复制标题
围产期可卡因暴露会减少新生大鼠自发活跃的中脑多巴胺神经元的数量。
DOI：
10.1002/syn.890170408
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Pitts,DK
中科院分区：
文献类型：
--
作者：
Wang,L;Pitts,DK
Evidence suggests that prenatal cocaine exposure in both rats (Dow-Edwards et al., 1990; Spear and Heyser, 1992) and humans (Needlman et al., 1993) may affect the subsequent development of central dopaminergic systems in offspring. Decreased cerebral spinal fluid levels of the dopamine (DA) metabolite, homovanillic acid (I-IVA), has been observed in cocaine-exposed newborns relative to unexposed controls (Needlman et al., 1993). Recently, electrophysiological studies conducted in the rat by Minabe et al.(1992) demonstrated that prenatal cocaine exposure (40 mg/kg/day, subcutaneous injections from gestational day eight to day twenty) resulted in a decreased number of spontaneously active midbrain DA-containing neurons in adult offspring. The present study examined the effects of perinatal exposure to cocaine, which was continually administered to the pregnant dam for sixteen days (50 mgkgl day by subcutaneous ALZET minipump) beginning on gestational day eight. The electrophysiological activity of midbrain dopamine neurons was examined in twoweek-old offspring following perinatal cocaine exposure. The physiological and pharmacological properties of two-week-old mesencephalic DA neurons differ significantly from those of adults (Pitts et al., 1990; Wang and Pitts, in press. This postnatal age also represents a time period during which normal developmentally regulated cell death is still occurring in the substantia nigra pars compacta (Janec and Burke, 1993). Therefore, the two-week-old mesencephalic DA neurons appear to be in a state of flux during normal postnatal development.Eight timed-pregnant Sprague-Dawley rats (Hilltop, Scottdale, PA) were housed in standard animal facilities three days prior to surgical implantation of ALZET minipumps (Alza Co., Palo Alto, CA). ALZET minipumps (model 2ML2; primed in saline for 24 hours at 37 C) containing either cocaine or the vehicle, deionized water, were implanted subcutaneously between the scapulae of pregnant dams under halothane anesthesia
