Determination of the luciferin contents in luminous and non-luminous beetles

Determination of the luciferin contents in luminous and non-luminous beetles
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DOI:
10.1271/bbb.80017
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发表时间:
2008-05-01
影响因子:
1.6
通讯作者:
Inouye, Satoshi
Inouye, Satoshi
中科院分区:
工程技术4区
文献类型:
--
作者:
Oba, Yuichi;Shintan, Takeru;Inouye, Satoshi

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采用高效液相色谱荧光检测法和荧光素与萤火虫荧光素酶的发光反应测定了夜光虫和非夜光虫中萤火虫荧光素的含量。发光性斑点和斑点动物含有不同数量的荧光素,范围在pmol到数百nmol之间,但在非发光斑点和斑点动物中检测到不同数量的荧光素。
The contents of firefly luciferin in luminous and non-luminous beetles were determined by the methods of HPLC with fluorescence detection and the luminescence reaction of luciferin and firefly luciferase. Luminous cantharoids and elaterids contained various amounts of luciferin in the range of pmol to hundreds of nmol, but no, luciferin was detected in the non-luminous cantharoids and elaterids.