EFFECTS OF INTERLEUKIN-1, INTERLEUKIN-2, INTERLEUKIN-4, INTERLEUKIN-6, INTERFERON-GAMMA AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR ON HUMAN VASCULAR ENDOTHELIAL-CELLS
EFFECTS OF INTERLEUKIN-1, INTERLEUKIN-2, INTERLEUKIN-4, INTERLEUKIN-6, INTERFERON-GAMMA AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR ON HUMAN VASCULAR ENDOTHELIAL-CELLS
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DOI：
10.1016/0165-2478(93)90078-g
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发表时间：
1993-02-01
影响因子：
4.4
通讯作者：
WOLNER, E
中科院分区：
文献类型：
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作者：
HOLZINGER, C;WEISSINGER, E;WOLNER, E
人血管内皮细胞 (HUVEC) 表现出多种免疫功能，即与 IFN-γ 孵育后表达 HLA II 类抗原或抗原呈递功能。据报道，HUVEC能够产生IL-1、IL-6、GM-CSF和具有免疫活性的花生四烯酸裂解产物。在我们的研究中，我们研究了各种细胞因子，即 IL-1、IL-2、IL-6、GM-CSF 和 IFN-gamma，是否确实改变 HUVEC 的增殖能力、van Willebrandt 因子 (vWF) 的产生和 MHC II 类抗原的表达。 HUVEC是通过胶原酶消化人脐静脉制备的。将单层细胞与不同浓度的细胞因子一起孵育24和48小时。 IFN-gamma 以剂量依赖性方式抑制 HUVEC [3H-3] 胸苷摄取。用 100 U IFN-gamma/ml 孵育 24 小时后可观察到增殖抑制 (40.1%)。 IL-1 是比 IFN-γ 更有效的 HUVEC 增殖抑制剂（10 U/ml 孵育 24 小时时为 54%，48 小时后为 48.4%）。 IL-6 和 GM-CSF 显示出对增殖的增强作用，分别为对照组的 226% 和 151%。 12小时潜伏期后的IFN-γ和24小时后的IL-1使vWF含量降低约30%。明亮的 MHC II 类表达仅由 IFN-γ 诱导。总之，一些免疫调节细胞因子可能在控制 HUVEC 增殖中发挥重要作用。
Human vascular endothelial cells (HUVEC) exhibit various immunological functions, i.e. expression of HLA class-II antigens after incubation with IFN-gamma or antigen presenting function. It has also been reported that HUVEC are able to produce IL-1, IL-6, GM-CSF and immunologically active cleavage products of arachidonic acid. In our study we investigated whether various cytokines, namely IL-1, IL-2, IL-6, GM-CSF and IFN-gamma, do alter the proliferative capacity of HUVEC, the production of van Willebrandt factor (vWF) and the expression of MHC class-II antigens. HUVEC were prepared by the collagenase digestion of human umbilical veins. Monolayers of cells were incubated with cytokines in different concentrations for 24 and 48 h. IFN-gamma inhibits the HUVEC [H-3]thymidine uptake in a dose-dependent manner. Suppression of proliferation (40.1%) could be observed after 24 h incubation with 100 U IFN-gamma/ml. IL-1 was a more effective inhibitor of HUVEC proliferation (54% at 10 U/ml and 24 h incubation and 48.4% after 48 h) than IFN-gamma. IL-6 and GM-CSF showed an increasing effect on proliferation with 226% and 151% of the control group, respectively. IFN-gamma after an incubation period of 12 h and IL-1 after 24 h reduced the vWF content by about 30%. Bright MHC class-II expression was induced only by IFN-gamma. In conclusion, some of the immunoregulative cytokines might play an important role in the control of HUVEC proliferation.