Serum retinol binding protein 4 is negatively related to beta cell function in Chinese women with non-alcoholic fatty liver disease: a cross-sectional study.
Serum retinol binding protein 4 is negatively related to beta cell function in Chinese women with non-alcoholic fatty liver disease: a cross-sectional study.
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中国女性非酒精性脂肪肝患者血清视黄醇结合蛋白 4 与 β 细胞功能负相关：一项横断面研究
DOI：
10.1186/1476-511x-12-157
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发表时间：
2013-10-27
影响因子：
4.5
通讯作者：
Gao X
中科院分区：
文献类型：
--
作者：
Yan H;Chang X;Xia M;Bian H;Zhang L;Lin H;Chen G;Zeng M;Gao X
观察非酒精性脂肪性肝病（NAFLD）患者血清视黄醇结合蛋白4（RBP 4）水平与胰岛β细胞功能的关系。106例经超声诊断为脂肪肝的患者（男/女：61/45;年龄47.44 ± 14.16岁）被纳入本横断面研究。排除已知患有糖尿病、慢性病毒性肝炎和过量饮酒的受试者。ELISA法检测血清RBP 4水平，Western印迹法检测血清RBP 4水平。用HOMA法测定所有受试者的β细胞功能，用高血糖钳夹法测定17例糖耐量正常者（男6例，女11例）的β细胞功能。男性血清RBP 4水平明显高于女性（55.96 ± 11.14vs45.87 ± 10.31 μg/ml，P < 0.001）。Pearson相关分析显示，女性血清RBP 4水平与空腹血糖（FBG）、HOMA-β、胰岛素第一时相分泌增量（1 PH）显著相关，而与年龄、BMI、腰围、WHR、收缩压（SBP）、舒张压（DBP）、TC、TG、HDL-c、LDL-c、2 h血糖、HOMA-IR、ALT、AST、γ-GT、肝脏脂肪含量（HFC）和胰岛素敏感性指数（ISI）。然而，在男性中，血清RBP 4水平与HDL-c、ALT、AST显著相关，但与上述任何其他参数无关。多元逐步回归分析显示，女性HOMA-IR和RBP 4与HOMA-β显著相关，男性HOMA-IR和BMI与HOMA-β显著相关。主要由肝脏和脂肪组织分泌的血清RBP 4可能参与了中国女性NAFLD患者β细胞功能障碍的发病机制。
To observe the relationship between serum retinol binding protein 4(RBP4) and β cell function in Chinese subjects with non-alcoholic fatty liver disease (NAFLD) and without known diabetes. 106 patients diagnosed as fatty liver by ultrasonography (M/F: 61/45; aged 47.44 ± 14.16 years) were enrolled in our current cross-sectional study. Subjects with known diabetes, chronic virus hepatitis and excessive alcohol consumption were excluded. Serum RBP4 was detected by ELISA and validated by quantitative Western blotting. β cell function were assessed by HOMA in all subjects and by hyperglycemic clamp in 17 normal glucose tolerance subjects (M = 6, F = 11). The levels of serum RBP4 in men were higher than that in women (55.96 ± 11.14 vs 45.87 ± 10.31 μg/ml, p < 0.001). Pearson’s correlation analysis demonstrated that in women, serum RBP4 levels were significantly associated with fasting blood glucose (FBG), HOMA-β, and increment of first phase insulin secretion (1PH), but not associated with age, BMI, waist circumference, WHR, systolic (SBP) and diastolic blood pressure (DBP), TC, TG, HDL-c, LDL-c, 2 h blood glucose, HOMA-IR, ALT, AST, γ-GT, hepatic fat content (HFC), and insulin sensitivity index (ISI). However, in men, serum RBP4 levels were significantly associated with HDL-c, ALT, AST, but not associated with any other parameters as mentioned above. A stepwise multiple linear regression analysis demonstrated that in women, HOMA-IR and RBP4 were significantly associated with HOMA-β, while in men, HOMA-IR and BMI were significantly variables associated with HOMA-β. Serum RBP4, secreted mainly by liver and adipose tissue, may involve in the pathogenesis of β cell dysfunction in Chinese women patients with NAFLD.